《厦门大学学报（自然科学版）》

为了研究对虾科(Penaeidae)对虾的系统发育关系,基于2种细胞核蛋白编码基因钾钠腺苷三磷酸α亚基(NaK)基因和磷酸烯醇丙酮酸激酶(PEPCK)基因,使用邻接(neighbor-jointing,NJ)法构建了9属16种对虾的系统发育树.研究结果有力地支持了1983年Burkenroad提出的三群体分类系统,除新对虾属处于进化树最先起源的位置外,系统发育树形成3个进化枝,其一为对虾属、明对虾属、滨对虾属、囊对虾属和沟对虾属,其二为仿对虾属和鹰爪虾属,其三为赤虾属,分别对应为Penaeini、Trachypenaeini和Parapenaeini 3个群体.基于核基因的系统发育树很好地解决了线粒体基因构建的系统发育树中对虾属和明对虾属进化关系不明确的问题,赤虾属的进化关系也很好地符合了形态学分类结果,因此可以作为对虾系统进化研究的重要补充手段与其他的研究方法联合应用.

In order to study the phylogenetic relationship of penaeid shrimps(Penaeidae),the phylogenetic tree of 16 species of penaeid shrimps from 9 genera was constructed using a neighbor-jointing method based on the nuclear protein-coding genes NaK(sodium-potassium ATPase α-subunit)and PEPCK(phosphoenolpyruvate carboxykinase).The result vigorously supports the three-tribe scheme proposed by Burkenroad in 1983 with three clades resolved:Penaeus sensu strict,Fenneropenaeus,Litopenaeus,Marsupenaeus and Melicertus as clade Ⅰ,Parapenaeopsis and Trachypenaeus as clade Ⅱ,and Metapenaeopsis as clade Ⅲ,corresponding to the Penaeini,Trachypenaeini,and Parapenaeini,respectively,except for Metapenaeus at the root of the phylogenetic tree.The phylogenetic tree of nuclear genes solves the obscure evolutionary relationship between Penaeus sensu stricto and Fenneropenaeus,which cannot be explained by the phylogenetic tree built based on mitochondrial genes.The phylogenetic relationship among the Metapenaeopsis is also well consistent with the result of the morphological classification.Accordingly,it can be used as an important complement to the phylogeny of penaeid shrimps,with the assistance of other methods.

引言
1 材料和方法
2 结果与分析
3 讨论

表1 对虾科16种对虾和披针单肢虾的NaK和PEPCK基因片段核苷酸组成<br/>Tab.1 Nucleotide compositions of NaK and PEPCK genes of 16 species of Penaeidae and S. lancifer%

表1 对虾科16种对虾和披针单肢虾的NaK和PEPCK基因片段核苷酸组成
Tab.1 Nucleotide compositions of NaK and PEPCK genes of 16 species of Penaeidae and S. lancifer%

表2 对虾科16 种对虾NaK和PEPCK基因的相对遗传距离矩阵<br/>Tab.2 Pairwise distance matrix of NaK and PEPCK genes among 16 species of Penaeidae

表2 对虾科16 种对虾NaK和PEPCK基因的相对遗传距离矩阵
Tab.2 Pairwise distance matrix of NaK and PEPCK genes among 16 species of Penaeidae

图1 基于NaK基因序列(a)、PEPCK基因序列(b)及NaK和PEPCK基因合并序列(c)构建的系统发育树<br/>Fig.1 Phylogenetic trees based on NaK gene sequences(a),PEPCK gene sequences(b)

图1 基于NaK基因序列(a)、PEPCK基因序列(b)及NaK和PEPCK基因合并序列(c)构建的系统发育树
Fig.1 Phylogenetic trees based on NaK gene sequences(a),PEPCK gene sequences(b)

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引言

1 材料和方法

2 结果与分析

3 讨论

学报简介

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引言

1 材料和方法

2 结果与分析

3 讨 论

学报简介

3 讨论