龙眼DlIKU1基因的克隆与功能分析
姜 帆1,田真真2,陈雪玮2,郑少泉1,陈 亮2*

(1.福建省农业科学院果树研究所,福建 福州 350003; 2.厦门大学 生命科学学院,厦门市植物遗传重点实验室,福建 厦门 361102)

龙眼; DlIKU1基因; 克隆; 生物信息学分析

Cloning and Functional Analysis of DlIKU1 Gene in Dimocarpus longan Lour.
JIANG Fan1,TIAN Zhenzhen2,CHEN Xuewei2,ZHENG Shaoquan1,CHEN Liang2*

(1.Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China; 2.Xiamen Key Laboratory for Plant Genetics,School of Life Sciences,Xiamen University,Xiamen 361102,China)

Dimocarpus longan Lour.; DlIKU1 gene; cloning; bioinformatic analysis

DOI: 10.6043/j.issn.0438-0479.201604101

备注

拟南芥(Arabidopsis thaliana)IKU1基因突变可以产生较小的种子.根据植物IKU1同源基因的高度保守性,设计引物,PCR扩增获得龙眼(Dimocarpus longan Lour.)同源基因DlIKU1的编码序列,其编码的蛋白含有VQ保守基序,与其他物种中同源蛋白的氨基酸序列存在较高的同源性; 由于龙眼遗传转化的限制,构建DlIKU1基因的超量表达载体并转化拟南芥iku1突变体,统计转基因植株子代种子的长宽变化,结果表明:龙眼DlIKU1基因的超量表达可以显著增加拟南芥种子的大小,说明龙眼DlIKU1基因可以影响种子发育.上述结果为研究龙眼种子发育机理提供了理论和实验依据,有助于龙眼产业的发展.

IKU1 gene mutation in Arabidopsis thaliana produced small seeds.The primers were designed according to the highly conservative sequence of IKU1 homologous genes. The longan DlIKU1 gene was obtained via PCR amplification.The amplified sequences of Longan DlIKU1,990 bp,contained VQ motif,and had high homology with the other species.Because of low efficiency of the longan genetic transformation,we constructed the overexpression vector of DlIKU1 gene for genetic transformation in Arabidopsis.Then we summarized changes in length and width of the progeny seed to study the function of DlIKU1 gene.The results showed that DlIKU1 gene might affect seed development to a certain extent.This study provided theoretical and experimental basis for the development of longan seed,and it will be helpful to the development of longan industry.

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