《厦门大学学报（自然科学版）》

为明晰低磷条件下内生真菌对台湾相思(Acacia confusa)叶绿素荧光特性的影响,探讨利用内生真菌提高台湾相思光合特性的可能性,从台湾相思中分离纯化4株强势内生真菌F(Filobasidium sp.)、I(Penicillium sp.)、E(Penicillium sp.)、N(Penicillium sp.)及FI混合菌(质量比1:1),并添加于4个供磷水平(0,10,20,40 mg/kg)的土培台湾相思幼苗盆钵中.结果表明:在适量供磷条件下(40 mg/kg),对照组(未接菌种)的叶绿素含量均总体低于各菌种处理组,菌株I、FI处理组的光合系统Ⅱ(PSⅡ)活性(Fv/Fo)、PSⅡ最大光能转换效率(Fv/Fm)总体略高于对照组; 轻度磷胁迫下(20 mg/kg),对照组的叶绿素含量始终低于菌株E、FI和F处理组,而不同处理组的Fv/Fo、Fv/Fm则总体差异不显著; 重度磷胁迫下(10 mg/kg),菌株F、E、N处理组的叶绿素含量总体较高,其中菌株E处理组的Fv/Fo、Fv/Fm始终高于对照组; 无磷处理下(0 mg/kg),菌株FI、I处理组在胁迫中后期的叶绿素含量较高,菌株I处理组的Fv/Fo、Fv/Fm 始终略高于对照组.综上,菌株F、FI处理对台湾相思叶绿素荧光特性的促进作用最佳,明确了适合低磷环境促进台湾相思叶绿素荧光特性的菌种,可为理解内生真菌对台湾相思光合特性的促进机制提供参考,亦为沿海台湾相思海岸防护林的持续性经营提供新思路.

Objective: Phosphorus is one of the important elements in plants, where it regulates photosynthesis by participating various physiological process. The goals of this work are to clarify the effect of endophytic fungi on the chlorophyll fluorescence characteristics of Acacia confusa Merr. under low-phosphorus conditions, and to explore the possibility of using endophytic fungi to improve the photosynthetic characteristics of A. confusa Merr..
Methods: Four strains of powerful endophytic fungi F (Filobasidium sp.), I (Penicillium sp.), E (Penicillium sp.), N (Penicillium sp.) and FI mixed bacteria (1:1) were isolated and purified from A. confusa Merr. and added to four phosphorus levels (0, 10, 20, 40 mg/kg) soil culture of A. confusa Merr. seedlings in pots. The indexes used in this study included chlorophyll content, photosynthetic system Ⅱ (PSⅡ) activity (Fv/Fo), and PSⅡ maximum light energy conversion efficiency (Fv/Fm).
Results: Under an appropriate amount of phosphorus supply (40 mg/kg), the chlorophyll content of the control group (non-inoculated) was overall lower than that of each strain treatment group, and the Fv/Fo and Fv/Fm of the strain I and FI treatment groups was slightly higher than the control group; under mild phosphorus stress (20 mg/kg), the chlorophyll content of the control group was lower than that of stain E and FI treatment groups, while the Fv/Fo and Fv/Fm were not significantly different among different treatment groups; under severe phosphorus stress (10 mg/kg), the chlorophyll contents of strains F, E, and N treatment groups were overall higher. For strain E and N treatment groups, the Fv/Fo and Fv/Fm were higher than those of the control group; under the treatment group without phosphorus (0 mg/kg), the chlorophyll contents of the strain FI and I treatment groups were higher at the middle and late stage of stress, and the Fv/Fo and Fv/Fm of the strain I treatment group were higher than those of the control group. Compared with the Fv/Fo of the seedlings at 30 d, 70 d, and 90 d, the Fv/Fo of the seedlings at 50 d in each group was significantly higher (P<0.05).
Conclusion: In this study, we used the endophytic fungi back inoculated to different low-phosphorus treated A. confusa Merr. seedlings, and analyzed the chlorophyll content and chlorophyll fluorescence parameters of the endophytic fungi and Acacia confusa Merr. symbionts to clarify the effect of the dominant endophytic fungi on the chlorophyll fluorescence characteristics of Acacia confusa Merr. under low phosphorus stress. Overall, our results showed that the strain F and FI had the best promotion effect on the chlorophyll fluorescence characteristics of A. confusa Merr. under low-phosphorus conditions. Our observations provide an important reference for improving the growth and photosynthetic characteristics of A. confusa Merr. coastal protection forests. However, only from the perspective of the promotion of photosynthetic characteristics of A. confusa Merr. by the dominant endophytic fungi, their growth could not be systematically improved in low-phosphorus habitats in southern China. Therefore, further studies are encouraged to focus on the promotion mechanism of endophytic fungi on inter-root acidity, organic acid secretion, phosphatase release and soil phosphorus activation of Acacia confusa Merr..

引言
1 材料与方法
2 结果与分析
3 讨论与结论

表1 不同内生真菌在不同试验天数及不同磷胁迫下对台湾相思幼苗叶片中叶绿素含量的影响<br/>Tab.1 Effects of different endophytic fungi on chlorophyll contents in the leaves of A.confusa seedlings under different experimental days and different phosphorus stress

表1 不同内生真菌在不同试验天数及不同磷胁迫下对台湾相思幼苗叶片中叶绿素含量的影响
Tab.1 Effects of different endophytic fungi on chlorophyll contents in the leaves of A.confusa seedlings under different experimental days and different phosphorus stress

表2 不同内生真菌在不同试验天数及不同磷胁迫下对台湾相思幼苗叶片PSⅡFv/Fo的影响<br/>Tab.2 Effects of different endophytic fungi on PSⅡFv/Fo in the leaves of A.confusa seedlings under different experimental days and different phosphorus stress

表2 不同内生真菌在不同试验天数及不同磷胁迫下对台湾相思幼苗叶片PSⅡFv/Fo的影响
Tab.2 Effects of different endophytic fungi on PSⅡFv/Fo in the leaves of A.confusa seedlings under different experimental days and different phosphorus stress

表3 不同内生真菌在不同试验天数及不同磷胁迫下对台湾相思幼苗叶片PSⅡFv/Fm的影响<br/>Tab.3 Effects of different endophytic fungi on the PSⅡ Fv/Fm in the leaves of A.confusa seedlings under different experimental days and different phosphorus stress

表3 不同内生真菌在不同试验天数及不同磷胁迫下对台湾相思幼苗叶片PSⅡFv/Fm的影响
Tab.3 Effects of different endophytic fungi on the PSⅡ Fv/Fm in the leaves of A.confusa seedlings under different experimental days and different phosphorus stress

表4 菌株种类、试验天数和胁迫条件对台湾相思叶绿素含量和叶绿素荧光参数影响的多因素方差分析<br/>Tab.4 Multivariate analysis of the influence of strain types,test days and stress conditions on the chlorophyll contents and chlorophyll fluorescence parameters of A.confusa

表4 菌株种类、试验天数和胁迫条件对台湾相思叶绿素含量和叶绿素荧光参数影响的多因素方差分析
Tab.4 Multivariate analysis of the influence of strain types,test days and stress conditions on the chlorophyll contents and chlorophyll fluorescence parameters of A.confusa

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备注

引言

1 材料与方法

2 结果与分析

3 讨论与结论

学报简介