内生真菌对杉木人工林下土壤酶活性的影响

(1.福建农林大学林学院,福建 福州 350002; 2.武夷学院生态与资源工程学院,福建 南平 354300)

杉木; 内生真菌; 土壤酶活性; 凋落叶; 质量损失率

Effects of endophytic fungi on soil enzyme activities underCunninghamia lanceolata plantation
LI Guanjun1,WU Chengzhen2*,LIANG Anjie1,HONG Tao1,CHEN Can1,XIE Anqiang1,HONG Wei1,LIN Yongming1,LI Jian1*

(1.College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China; 2.College of Ecology and Resource Engineering,Wuyi University,Nanping 354300,China)

Cunninghamia lanceolata; endophytic fungi; soil enzyme activity; leaf litter; mass loss rate

DOI: 10.6043/j.issn.0438-0479.201808011

备注

通过筛选能够提高土壤酶活性的菌株及添加方式,探讨内生真菌对杉木(Cunninghamia lanceolata)人工林下土壤酶活性的影响,以促进叶凋落物分解.采用3株杉木内生真菌青霉菌(Penicillium sp.)CG2(A菌)、黄色镰刀菌(Fusarium culmorum)AY13(B菌)、踝节霉菌(Talaromyces sp.)AJ14(C菌)的单菌株和混菌株,按不同方式(菌丝、菌液)浇施于装有杉木凋落叶和土壤的盆钵内,在处理10,60,120 d后分别取样,测定土壤酶活性和凋落叶质量的变化情况.结果表明:多酚氧化酶活性在混菌丝处理下始终保持在较高水平,菌丝和菌液处理均提高了脲酶和酸性磷酸酶活性,蔗糖酶活性前期(10 d)高于后期(120 d).C菌液处理下(120 d)纤维素酶、木质素过氧化物酶和酸性磷酸酶活性均最高,与对照存在显著差异(p<0.05),分别比对照高56.46%,43.97%和163.65%; BC混菌丝处理下(120 d)多酚氧化酶活性最高,比对照高36.75%(p<0.05); AB混菌丝处理下(10 d)蔗糖酶活性最高,比对照高162.90%(p<0.05); AC混菌液处理下(120 d)脲酶活性最高,比对照高69.78%(p<0.05).凋落叶质量损失率与纤维素酶、脲酶、酸性磷酸酶、木质素过氧化物酶以及混菌处理的多酚氧化酶活性变化趋势相同,菌株种类、添加方式、分解时间及其交互作用对酶活性有显著影响.综上所述,C菌液能最有效地提高纤维素酶、木质素过氧化物酶和酸性磷酸酶活性,BC和AB混菌丝分别对提高多酚氧化酶和蔗糖酶活性最有效,AC混菌液则能最有效地提高脲酶活性.

By screening the strains and the adding methods to increase soil enzyme activities,the effects of endophytic fungi on soil enzyme activities under Chinese fir(Cunninghamia lanceolata)plantation were investigated to promote the decomposition of leaf litter.The single strain and mixed strains of three endophytic fungi of C. lanceolata(Penicillium sp.CG2(A),Fusarium culmorum AY13(B),Talaromyces sp.AJ14(C))were poured into the experimental pots in two ways(mycelia and bacterial solution),and were sampled after 10,60,and 120 d for determination of changes in soil enzyme activity and litter quality.The results showed that the laccase activity was maintained at a high level under the treatment of mixed mycelia.The activities of urease and acid phosphatase were improved under both mycelia and bacterial solution treatments.The activity of sucrase in the early stage(10 d)was higher than that in the later stage(120 d).Cellulase,lignin peroxidase and acid phosphatase activities were significantly different from the control under the treatment of bacterial solution of strain C(120 d)(p<0.05),which were 56.46%,43.79% and 163.65% higher than that of the control,respectively.The laccase activity was 36.75% higher than that of the control under BC mixed mycelia treatment(120 d,p<0.05).The sucrase activity under AB mixed mycelia treatment was 162.90% higher than that of the control(10 d,p<0.05).The urease activity under AC mixed bacterial solution was 69.78% higher than that of the control(120 d,p<0.05).The change of leaf litter mass loss rate was the same as that of cellulase, urease,acid phosphatase and lignin peroxidase activities,as well as the laccase activity under mixed bacterial treatment.The fungus type,addition mode,decomposition time,and the interaction between the three factors have significant effects on enzyme activities.In summary,the bacterial solution of strain C could increase the activities of cellulase, lignin peroxidase and acid phosphatase most efficiently,the mycelia of BC and AB mixed strains could increase the activities of laccase and sucrase most efficiently,and the bacterial solution of AC mixed strains could increase the urease activity most efficiently.