盐度对水培海马齿生长和生理生化因子的影响

(1.福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室,福建 厦门 361013; 2.福建省海洋生物资源开发利用协同创新中心,福建 厦门 361013)

海马齿; 盐度; 生理生化响应; 生态修复

Effects of salinity on growth,physiology and biochemistry of hydroponic Sesuvium portulacastrum
LI Weilin1,2,LUO Donglian1,2*,YANG Fang1,2,ZHENG Huidong1,2,LIN Qi1

(1.Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,Fisheries Research Institute of Fujian,Xiamen 361013,China; 2.Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological R

Sesuvium portulacastrum; salinity; physiological biochemical responses; ecological remediation

DOI: 10.6043/j.issn.0438-0479.201803036

备注

通过水培实验模拟生态浮床,研究海马齿(Sesuvium portulacastrum)在不同盐度条件下的生长及生理生化响应特征,探讨海马齿盐度适应性和海马齿生态浮床修复技术推广的适宜盐度范围.结果表明:在中、低盐度(0~20)条件下,海马齿生长几乎不受影响,而在高盐度(25~35)水体中,其生长受到明显胁迫,其中最高盐度(35)处理组中部分海马齿茎节出现腐烂溃败的现象,苗种成活率下降; 新根数为低盐度(0~10)处理组>中盐度(15和20)处理组>高盐度(25~35)处理组,且高盐度处理组新根呈现粗、短特征; 培养40 d后,海马齿植株鲜质量、干质量、相对生长量、水分含量、根系活力和根质量随盐度升高均呈现先上升后下降的趋势,各指标最大值均落在盐度5~15范围内,其中根质量和根系活力的最大值均出现在盐度15处理组,表明适当的盐度可以促进水培海马齿的根系发育; 除可溶性糖外,海马齿叶片中丙二醛和叶绿素含量受盐度变化的影响较小,不同盐度组间无显著差异(p>0.05),暗示海马齿可利用渗透压调节机制抵抗环境盐胁迫,以保证细胞内其他生理生化指标维持较稳定的状态.综合比较可初步确定海马齿的适宜生长盐度范围为0~15.

Using hydroponics to mimic ecological floating bed,the growth,physiological and biochemical responses of hydroponic Sesuvium portulacastrum under different salinity conditions were studied,to explore its salinity adaptability and suitable salinity range for promotion of the ecological floating bed remediation technology.Results showed that S.portulacastrum could grow fast and healthy at medium and low salinity(0-20),but the growth was significantly restricted at high salinity(25-30).In the highest salinity(35)treatment group,some stems rotted and collapsed,and the survival rate of plant decreased obviously.New root number in different treatment groups showed as follows: low salinity(0-10)treatment group>medium salinity(15-20)treatment group>high salinity(25-35)treatment group,and roots in high salinity treatment group were thicker and shorter.The fresh mass,dry mass,relative growth yield,water content,root activity and root mass of S.portulacastrum increased first and then decreased with the increase of salinity after 40 d hydroponic cultivation,and the maximum value of each index fell within salinity range from 5 to 15.The salinity 15 group showed the maximum values of root activity and root mass,which indicated that appropriate salinity could promote root development of S.portulacastrum.Except for soluble sugar,the content of malondialdehyde and chlorophyll in leaves of S.portulacastrum were not significantly affected by salinity(p>0.05),suggesting that osmotic regulation ability could be used by S.portulacastrum for salt tolerance,so that to maintain a relatively stable state of other physiological and biochemical indicators in cell.Comprehensive comparison suggests the optimum salinity for growth of S.portulacastrum is from 0 to 15.