对流实时荧光定量PCR系统设计

(1.厦门大学公共卫生学院,福建 厦门 361102; 2厦门大学信息科学与技术学院, 福建 厦门 361005; 3.国家传染病诊断试剂与疫苗工程技术研究中心(厦门大学),福建 厦门 361102)

实时荧光定量PCR; 自然对流PCR; 双通道; 快速扩增

Design on Real-time Fluorescence Quantitative PCR System Based on Natural Convection
WENG Zhenyu1,MIN Xiaoping2,3*,WANG Hai2,ZHUO Zhihao3,GE Shengxiang3

(1.Schoolof Public Health,Xiamen University,Xiamen 361102,China; 2.School of Information Science and Engineering,Xiamen University,Xiamen 361005,China; 3.National Institute of Diagnostics and Vaccine Development in Infection Diseases(Xiamen University),Xi

real-time fluorescence quantitative PCR; natural convection PCR; two-channel structure; rapid amplification

DOI: 10.6043/j.issn.0438-0479.201705041

备注

设计了一种结合实时荧光定量技术和自然对流技术的PCR系统.主要介绍该系统的双通道荧光激发与检测结构及其工作原理,系统中使用光纤作为光路的传输媒介,采用电荷耦合元件(CCD)工业相机采集荧光信号变化,并自行设计电路和软件驱动整套系统正常运行.最后通过对梯度稀释的巨细胞病毒(CMV)的基因模板进行扩增,展示了该系统可用于快速扩增和实时定量检测的潜力.

A PCR system with real-time fluorescent quantitative technology and natural convection technology was designed.This paper mainly introduced the two-channels structure and the work principle of fluorescence excitation and fluorescence detection.In the system,fiber optics were used as an optical transmission medium and a charge coupled device(CCD)camera was applied to collect the change of fluorescent signal.To ensure that the system work steadily,the circuit and software were designed independently.Finally,we showed the system's potential of rapid nucleic acid amplification and real-time quantitation by amplifying gradient-diluted gene template of cytomegalovirus(CMV).