《厦门大学学报（自然科学版）》

合成聚肽(synthetic polypeptides)在靶向递送、组织工程和再生医学领域表现独特,但其僵硬的主链构象导致难以用电纺的方法制备聚肽纳米纤维,而且后续的表面改性通常会破坏纤维的形貌,因而限制了这类材料的应用.为此,使用聚肽和聚己内酯(PCL)共混电纺的方法制备了一类新型的纳米纤维,并利用巯-炔(Thiol-Yne)光点击反应快速、高效、无损伤地对其表面进行改性.采用扫描电镜(SEM)、傅里叶变换红外(FT-IR)光谱和X射线光电子能谱(XPS)对其进行表征,结果显示:共混纤维的直径和形貌受聚肽含量的影响,而Thiol-Yne表面改性和后处理对纤维的形貌没有影响,这说明所制备的纤维的稳定性很好; 利用温和的Thiol-Yne光点击反应可以成功地将氨基引入到纤维表面.

Synthetic polypeptides are unique biomaterials in the field of drug/gene delivery,tissue engineering and regenerative medicine.But electrospinning nanofibers used for tissue engineering are hard to be fabricated as a result of the rigid backbone of polypeptides caused by their α-helix and β-sheet conformations.In addition,the subsequent surface modifications of electrospinning nanofibers probably destroy the nanofibers.Thus,the fabrication and application of the electrospinning nanofibers of synthetic polypeptides are limited.In this work,electrospinning polypeptide/polycaprolactone(PCL)blend nanofibers were fabricated and then undergone surface modification by thiol-yne click chemistry,a fast non-destructive and highly effective access.Results of scanning electron microscope(SEM)and Fourier transform-infrared(FT-IR)spectroscopy showed that diameter and orientation of the nanofibers were determined by the polypeptide contents,and the surface modification did not destroy the surface morphology.X-ray photoelectron spectroscopy(XPS)confirmed the amine modification of the surface by the gentle thiol-yne method.

引言
1 实验部分
2 结果与讨论
3 结论

图1 含聚肽链段的三嵌段共聚物PPLG-PPO-PPLG及其侧链接枝聚合物的合成路线<br/>Fig.1 The synthetic route to prepare PPLG-PPO-PPLG triblock copolymers and their side-chain modification

图1 含聚肽链段的三嵌段共聚物PPLG-PPO-PPLG及其侧链接枝聚合物的合成路线
Fig.1 The synthetic route to prepare PPLG-PPO-PPLG triblock copolymers and their side-chain modification

图2 PPLG-PPO-PPLG(a)和PPLGgCAH-PPO-PPLGgCAH(b,c)的1H-NMR谱图<br/>Fig.2 1H-NMR spectra of PPLG-PPO-PPLG(a)and PPLGgCAH-PPO-PPLGgCAH(b,c)

图2 PPLG-PPO-PPLG(a)和PPLGgCAH-PPO-PPLGgCAH(b,c)的1H-NMR谱图
Fig.2 1H-NMR spectra of PPLG-PPO-PPLG(a)and PPLGgCAH-PPO-PPLGgCAH(b,c)

$图3 不同聚肽质量分数下聚肽/PCL共混电纺纳米纤维的SEM图<br/>Fig.3 SEM images of electrospinning polypeptide/PCL blend nanofibers at different polypeptide mass fraction$

图3 不同聚肽质量分数下聚肽/PCL共混电纺纳米纤维的SEM图
Fig.3 SEM images of electrospinning polypeptide/PCL blend nanofibers at different polypeptide mass fraction

$图4 聚肽质量分数为2.25%时共混纤维在表面改性前(a)和改性后(b)的SEM图<br/>Fig.4 SEM images of blend nanofibers at 2.25%(mass fraction)polypeptide before(a)and after(b)the surface modification$

图4 聚肽质量分数为2.25%时共混纤维在表面改性前(a)和改性后(b)的SEM图
Fig.4 SEM images of blend nanofibers at 2.25%(mass fraction)polypeptide before(a)and after(b)the surface modification

图5 聚肽/PCL共混电纺纳米纤维表面在改性前后的ATR-FT-IR谱图<br/>Fig.5 ATR-FT-IR spectra of the electrospinning polypeptide/PCL blend nanofibers before and after the surface modification

图5 聚肽/PCL共混电纺纳米纤维表面在改性前后的ATR-FT-IR谱图
Fig.5 ATR-FT-IR spectra of the electrospinning polypeptide/PCL blend nanofibers before and after the surface modification

图6 聚肽/PCL共混电纺纳米纤维表面改性前后的XPS图<br/>Fig.6 XPS of the electrospinning polypeptide/PCL blend nanofibers before and after the surface modification

图6 聚肽/PCL共混电纺纳米纤维表面改性前后的XPS图
Fig.6 XPS of the electrospinning polypeptide/PCL blend nanofibers before and after the surface modification

图7 PPLG-PPO-PPLG的热失重曲线<br/>Fig.7 Thermal loss weight analysis of the PPLG-PPO-PPLG

图7 PPLG-PPO-PPLG的热失重曲线
Fig.7 Thermal loss weight analysis of the PPLG-PPO-PPLG

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备注

引言

1 实验部分

2 结果与讨论

3 结论

学报简介

备注

引言

1 实验部分

2 结果与讨论

3 结 论

学报简介

3 结论