苯甲醛L-半胱氨酸席夫碱联合柞木木醋液的杀虫活性
贾玉龙1,郑 静1,陈振雄1,南秀杰2,孙 悦3,陈清西1*

(1.厦门大学生命科学学院,福建 厦门 361102; 2.辽宁省抚顺市新抚区环保局, 辽宁 抚顺 113000; 3.辽宁清远环境能源科技有限公司,辽宁 抚顺 113006)

L-半胱氨酸席夫碱; 木醋液; 酪氨酸酶; 杀虫剂; 毒理学

Insecticidal Activity for Benzaldehyde L-Cysteine Schiff Base Combined with Xylosma Wood Vinegar
JIA Yulong1,ZHENG Jing1,CHEN Zhenxiong1,NAN Xiujie2,SUN Yue3,CHEN Qingxi1*

(1.School of Life Sciences,Xiamen University,Xiamen 361102,China; 2.Environmental Bureau of Xinfu District in Fushun of Liaoning Province,Fushun 113000,China; 3.Qingyuan Environment Energy Technology Co.,Ltd. of Liaoning Province,Fushun 113006,China)

L-cysteine Schiff base; wood vinegar; tyrosinase; insecticide; toxicology

DOI: 10.6043/j.issn.0438-0479.201602101

备注

木醋碱杀虫剂是一种基于木炭副产物木醋液的绿色杀虫剂,是将精馏木醋液、苯甲醛L-半胱氨酸席夫碱、水杨酸按照一定比例配制而成的.以酪氨酸酶筛选模型研究了苯甲醛L-半胱氨酸席夫碱、精馏木醋液、木醋碱杀虫剂对酪氨酸酶的抑制作用,其导致酪氨酸酶活性的半抑制质量浓度(IC50)分别为15,2.53,0.53 mg/mL.探讨了木醋碱杀虫剂对小菜蛾(Plutella xylostella)、棉铃虫(Helicoverpa armigera)、玉米螟(Pyrausta nubilalis)幼虫的杀虫活性,结果表明木醋碱杀虫剂中苯甲醛L-半胱氨酸席夫碱质量浓度达到15 mg/mL时即可对小菜蛾、棉铃虫、玉米螟二龄幼虫达到很好的杀灭效果.急性毒理实验表明木醋碱杀虫剂实际无毒.慢性毒理研究按照3 g/kg给药量连续灌胃3个月,昆明小鼠的肝脏和肾脏并不存在毒理性变化,表明木醋碱杀虫剂没有慢性毒性.综上所述,木醋碱杀虫剂不仅对酪氨酸酶有很好的抑制作用,还对小菜蛾、棉铃虫、玉米螟等常见农业害虫具有较强的毒杀活性,且实际无毒,具有良好的农业应用前景.

The wood vinegar was based on by-product in the process of firing Xylosma charcoal.The wood vinegar base insecticide was a new green type insecticide.We mixed the rectificated wood vinegar,benzaldehyde L-cysteine Schiff base and salicylic acid to make the wood vinegar base insecticide proportionally.The inhibition on tyrosinase in screening model of benzaldehyde L-cysteine Schiff base,rectificated wood vinegar and the wood vinegar base insecticide were determined.The half maximal inhibitory concentration(IC50)were 15,2.53,0.53 mg/mL,respectively.Additionally,the insecticidal effects on Plutella xylostella,Pyrausta nubilalis and Helicoverpa armigera of wood vinegar base insecticide were investigated.The results showed that the wood vinegar base insecticide could effectively kill the second-stage juveniles of P. xylostella,P. nubilalis and H. armigera. The results showed that the wood vinegar base insecticide had insecticidal effect.The acute toxicological results showed that the wood vinegar base insecticide had no toxicity.In the chronic toxicological experiment,the group was given a gavage of 3 g/kg with the wood vinegar base insecticide in 3 months.We took the liver and kidney in paraffin section and HE staining after executing the Kunming mouse with cervical dislocation method.The results showed that the wood vinegar base insecticide had no chronic toxicity.In conclusion,the wood vinegar base insecticide could not only inhibit the tyrosinase acitivity,but also show good insecticidal effects on P. xylostella,P. nubilalis and H. armigera with low toxicity.The wood vinegar base insecticide had shown good application prospects in agricultural areas.

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