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[1]张财亮,黄 贝,熊 静,等.拟穴青蟹两个Crustin新变体的克隆与表达分析[J].厦门大学学报(自然科学版),2019,58(04):505-515.[doi:10.6043/j.issn.0438-0479.201902012]
 ZHANG Cailiang,HUANG Bei,XIONG Jing,et al.Cloning and expression analysis of two novel Crustin isoforms from Scylla Paramamosain[J].Journal of Xiamen University(Natural Science),2019,58(04):505-515.[doi:10.6043/j.issn.0438-0479.201902012]
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
58卷
期数:
2019年04期
页码:
505-515
栏目:
研究论文
出版日期:
2019-07-28

文章信息/Info

Title:
Cloning and expression analysis of two novel Crustin isoforms from Scylla Paramamosain
文章编号:
0438-0479(2019)04-0505-11
作者:
张财亮1黄 贝1熊 静1陈芳奕2黄文树123*
1.集美大学水产学院,福建省海洋生物资源开发利用协同创新中心,福建 厦门 361021; 2.海洋生物制备技术国家地方联合工程实验室,福建 厦门 361102; 3.农业部东海海水健康养殖重点实验室,福建 厦门 361021
Author(s):
ZHANG Cailiang1HUANG Bei1XIONG Jing1CHEN Fangyi2HUANG Wenshu123*
1.College of Fisheries,Fujian Collaborative Innovation Center forExploitation and Utilization of Marine Biological Resources, Jimei University,Xiamen 361021,China; 2.State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology,Xiamen
关键词:
抗菌肽 Crustin 基因克隆 基因组 表达分析 拟穴青蟹 䥺Symbol`@@
Keywords:
antimicrobial peptides Crustin gene cloning genomes expression analysis Scylla paramamosain
分类号:
Q 786
DOI:
10.6043/j.issn.0438-0479.201902012
文献标志码:
A
摘要:
Crustins是一类广泛存在于甲壳动物中富含半胱氨酸的阳离子抗菌肽.该研究从拟穴青蟹(Scylla Paramamosain)中克隆获得两个Crustin新变体,分别命名为SpCrus1b和SpCrus2b.分析显示两者的前体肽包含N-端信号肽和C-端成熟肽两部分,成熟肽均含有12个位置高度保守的半胱氨酸.SpCrus1b在信号肽和乳清酸蛋白(WAP)结构域之间存在一个半胱氨酸富集区,属于典型的Ⅰ型Crustins; SpCrus2b除具有SpCrus1b的结构特征外,在近N-端额外含有一个甘氨酸富集区,属于典型的Ⅱ型Crustins.基因组结构分析显示SpCrus1b的基因组DNA序列为4个外显子/3个内含子结构,而SpCrus2b的基因组DNA序列为2个外显子/1个内含子结构.两者均主要在鳃中表达,且在脂多糖(LPS)刺激24 h和聚肌胞苷酸(PolyI:C)刺激12 h后表达量均极显著上调(p<0.01),表明SpCrus1b和SpCrus2b参与了拟穴青蟹抗细菌和抗病毒的先天免疫过程.
Abstract:
Crustins,widely exist in crustaceans,are cationic cysteine-rich antimicrobial peptides(AMPs).In this study,two novel Crustin isoforms were identified from Scylla paramamosain,namelySpCrus1b and SpCrus2b.The analysis showed a signal peptide at the N-terminus and a mature peptide at the C-terminus in both propeptides,and there were 12 cysteine residues at highly conserved positions found in mature peptides.In SpCrus1b,there was a cysteine-rich region(CRR)between the signal peptide and whey acidic protein(WAP)domain,showing a typical character of type ⅠCrustins.Compared with SpCrus1b,SpCrus2b possessed an extra glycine-rich region(GRR)at the N-terminus,which was proposed as type Ⅱ Crustins.In addition,the genomic organization analysis revealed that SpCrus1b had 4 exons/3 introns, while SpCrus2b had 2 exons/1 intron.Both SpCrus1b and SpCrus2b were highly expressed in gills and could be significantly up-regulated(p<0.01)after 24 h lipopolysaccharide(LPS)challenge or 12 h polyinosinic-polycytidylic acid(PolyI:C)challenge. The results suggested that SpCrus1b and SpCrus2b were potential AMPs,which played an important role in the innate immune process of S. paramamosain against bacterial and viral infections.

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备注/Memo

备注/Memo:
收稿日期:2019-02-16录用日期:2019-04-14
基金项目:国家自然科学基金(U1805233,31172438); 海洋生物制备技术国家地方联合工程实验室(厦门大学)开放课题基金(201807)
*通信作者:wshuang@jmu.edu.cn
更新日期/Last Update: 1900-01-01