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[1]刘国强,阿尔新·哈布丁,李小俊,等.黑果枸杞根际土样来源Salininema属菌株438HGGQ3-1T的鉴定[J].厦门大学学报(自然科学版),2019,58(02):269-275.[doi:10.6043/j.issn.0438-0479.201806013]
 LIU Guoqiang,HABDEN Arxin,LI Xiaojun,et al.Identification of a Salininema sp.438HGGQ3-1T from rhizosphere soil of Lycium ruthenicum[J].Journal of Xiamen University(Natural Science),2019,58(02):269-275.[doi:10.6043/j.issn.0438-0479.201806013]
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
58卷
期数:
2019年02期
页码:
269-275
栏目:
研究论文
出版日期:
2019-03-27

文章信息/Info

Title:
Identification of a Salininema sp.438HGGQ3-1T from rhizosphere soil of Lycium ruthenicum
文章编号:
0438-0479(2019)02-0269-07
作者:
刘国强1阿尔新·哈布丁2李小俊34孙承航3郑 勇1旭格拉·哈布丁1*
1.新疆师范大学生命科学学院,新疆干旱区植物逆境生物学实验室,新疆 乌鲁木齐 830054; 2.新疆自治区人民医院小儿外科,新疆 乌鲁木齐 830001; 3.中国医学科学院&北京协和医学院医药生物技术研究所,北京 100050; 4.河北北方学院医学检验学院,河北 张家口 075000
Author(s):
LIU Guoqiang1HABDEN Arxin2LI Xiaojun 34SUN Chenghang 3ZHENG Yong1HABDEN Xugela1*
1.Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology,College of Life Science,Xinjiang Normal University,Urumqi 830054,China; 2.Department of Pediatric Surgery,Xinjiang Autonomous Region People’s Hospital,Urumqi 830001,China; 3.Institute of Medicinal Biotechnology,Chinese Academy of Medical Science & Peking Union Medical College,Beijing 100050,China; 4.College of Laboratory Medical Science,Hebei North University,Zhangjiakou 075000,China
关键词:
嗜盐放线菌 多相分类学 根际土样 Salininema 黑果枸杞
Keywords:
halophilic actinobacterium polyphasic taxonomy rhizosphere soil Salininema Lycium ruthenicum
分类号:
Q 93-331
DOI:
10.6043/j.issn.0438-0479.201806013
文献标志码:
A
摘要:
采用多相分类学的方法,即通过表型、化学分类和分子分类特征,并综合系统发育分析结果,探究我国新疆库尔勒地区黑果枸杞(Lycium ruthenicum)根际土样来源菌株438HGGQ3-1T的分类学地位.结果显示:菌株438HGGQ3-1T为革兰氏染色阳性,可形成广泛分枝状营养菌丝、略带弯曲的丝状气生菌丝和末端平截的杆状分生孢子,最适生长条件为28~37 ℃、pH 7.0~7.5和2%~12%(质量分数)NaCl.该菌株的氧化酶活性呈阴性,过氧化氩酶活性呈阳性,以内消旋2,6-二氨基庚二酸作为特征性二氨基酸,以葡萄糖和核糖作为特征性全细胞糖,细胞磷脂包含磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇甘露糖苷、磷脂酰肌醇和2种含糖的磷脂,主要的甲基萘醌为MK-10(H4)、MK-9(H4)和MK-10(H2),主要脂肪酸为anteiso-C17:0、anteiso-C15:0和iso-C16:0.菌株438HGGQ3-1T与糖霉菌科Salininema proteolyticum Miq-4TParaglycomyces xinjiangensis TRM 49201TSalilacibacter albus J11Y309T同源性较高,对应的16S rRNA基因序列相似度分别为99.79%,99.67%和96.00%,与S.proteolyticum IBRC-M 10908TP.xinjiangensis CCTCC AA 2013002T的DNA-DNA杂交值均高于70%.综上分析表明菌株438HGGQ3-1TS.proteolyticum.
Abstract:
A halophilic actinobacterium,designated as strain 438HGGQ3-1T,was isolated from a rhizosphere soil sample of the medicinal plant Lycium ruthenicum.In the present work,the taxonomic position of strain 438HGGQ3-1T was elucidated.Phenotypic,chemotaxonomic characterizations and genotypic and phylogenetic analyses were performed in this study.The results showed that cells of strain 438HGGQ3-1T were Gram-positive,and formed extensive branched substrate mycelium,slightly twisted aerial mycelia and chains of square-ended conidia.Cells grew optimally at temperature of 28 ℃ to 37 ℃,pH of 7.0 to 7.5 and in the presence of 2% to 12%(by mass)NaCl.The isolate was negative for oxidase activity and positive for catalase activity.It contained 2,6-diaminopimelic acid as the diagnostic diamino acid and glucose and ribose as the whole-cell sugars.The phospholipids consisted of phosphatidylethanolamine,diphosphatidylglycerol,phosphatidylglycerol,phosphatidylinositol mannoside,phosphatidylinositol and two phosphoglycolipids.The major menaquinones were MK-10(H4),MK-9(H4)and MK-10(H2).The major fatty acids were anteiso-C17:0,anteiso-C15:0 and iso-C16:0.It shared the highest levels of 16S rRNA gene sequence similarity with Salininema proteolyticum Miq-4T(99.79%),Paraglycomyces xinjiangensis TRM 49201T(99.67%)and Salilacibacter albus J11Y309T(96.00%).The levels of DNA-DNA relatedness with S.proteolyticum IBRC-M 10908T and P.xinjiangensis CCTCC AA 2013002T were both above 70%.In summary,the strain 438HGGQ3-1T was identified as a strain of the species S.proteolyticum.A halophilic actinobacterium,designated as strain 438HGGQ3-1T,was isolated from a rhizosphere soil sample of the medicinal plant Lycium ruthenicum.In the present work,the taxonomic position of strain 438HGGQ3-1T was elucidated.Phenotypic,chemotaxonomic characterizations and genotypic and phylogenetic analyses were performed in this study.The results showed that cells of strain 438HGGQ3-1T were Gram-positive,and formed extensive branched substrate mycelium,slightly twisted aerial mycelia and chains of square-ended conidia.Cells grew optimally at temperature of 28 ℃ to 37 ℃,pH of 7.0 to 7.5 and in the presence of 2% to 12%(by mass)NaCl.The isolate was negative for oxidase activity and positive for catalase activity.It contained 2,6-diaminopimelic acid as the diagnostic diamino acid and glucose and ribose as the whole-cell sugars.The phospholipids consisted of phosphatidylethanolamine,diphosphatidylglycerol,phosphatidylglycerol,phosphatidylinositol mannoside,phosphatidylinositol and two phosphoglycolipids.The major menaquinones were MK-10(H4),MK-9(H4)and MK-10(H2).The major fatty acids were anteiso-C17:0,anteiso-C15:0 and iso-C16:0.It shared the highest levels of 16S rRNA gene sequence similarity with Salininema proteolyticum Miq-4T(99.79%),Paraglycomyces xinjiangensis TRM 49201T(99.67%)and Salilacibacter albus J11Y309T(96.00%).The levels of DNA-DNA relatedness with S.proteolyticum IBRC-M 10908T and P.xinjiangensis CCTCC AA 2013002T were both above 70%.In summary,the strain 438HGGQ3-1T was identified as a strain of the species S.proteolyticum.

参考文献/References:

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[26] CHUN J,RAINEY F A.Integrating genomics into the taxonomy and systematics of the bacteria and archaea[J].Int J Syst Evol Microbiol,2014,64(2):316-324.[1] STACKEBRANDT E,RAINEY F A,WARD-RAINEY N L.Proposal for a new hierarchic classification system,Actinobacteria classis nov.[J].Int J Syst Bacteriol,1997,47:479-491.
[2] NIKOU M M,RAMEZANI M,AMOOZEGAR M A,et al.Salininema proteolyticum gen.nov.,sp.nov.,a halophilic rare actinomycete isolated from wetland soil,and emended description of the family Glycomycetaceae[J]. Int J Syst Evol Microbiol,2015,65(10):3727-3733.
[3] LUO X X,HAN X X,ZHANG F,et al.Paraglycomyces xinjiangensis gen.nov.,sp.nov.,a halophilic actinomycete[J].Int J Syst Evol Microbiol,2015,65(11):4263-4269.
[4] LI X X,LIU J M,WU Y,et al.Description of Salilacibacter albus gen.nov.,sp.nov.,isolated from a dried salt lake and reclassification of Paraglycomyces xinjiangensis Luo et al.2015 as a later heterotypic synonym of Salininema proteolyticum Nikou et al.2015 with emended descriptions of the genus Salininema and Salininema proteolyticum[J].Int J Syst Evol Microbiol,2016,66(7):4039-4045.
[5] SHIRLING E B,GOTTLIEB D.Methods for characterization of Streptomyces species[J].Int J Syst Bacteriol,1966,16(7):313-340.
[6] 杜慧竟,苏静,余利岩,等.药用植物内生放线菌的分离和生物学特性[J].微生物学报,2013(1):123-125.
[7] WILLIAMS S T,GOODFELLOW M,ALDERSON G,et al.Numerical classification of Streptomyces and related genera[J].J Gen Appl Microbiol,1983,129(6):1743-1813.
[8] 徐丽华,李文均,刘志恒,等.放线菌系统学:原理、方法及实践[M].北京:科学出版社,2006:40-53.
[9] STANECK J L,ROBERTS G D.Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography[J].Appl Microbiol,1974,28(2):226-231.
[10] HASEGAWA T,TAKIZAWA M,TANIDA S.A rapid analysis for chemical grouping of aerobic actinomycetes[J].J Gen Appl Microbiol,1983,29:319-322.
[11] COLLINS M D,PIROUZ T,GOODFELLOW M,et al.Distribution of menaquinones in actinomycetes and corynebacteria[J].J Gen Microbiol,1977,100(2):221-230.
[12] GUO L,TUO L,HABDEN X,et al.Allosalinactinospora lopnorensis gen.nov.,sp.nov.,a new menber of family Nocardiopsaceae isolated from soil[J].Int J Syst Evol Microbiol,2015,65(1):206-213.
[13] MINNIKIN D E,O’DONNELL A G,GOODFELLOW M,et al.An integrated procedure for the extraction of isoprenoid quinones and polar lipids[J].J Microbiol Methods,1984,2(5):233-241.
[14] SASSER M.Identification of bacteria by gas chromatography of cellular fatty acids:MIDI technical note 101[R].Newark:MIDI Inc,1990.
[15] LIU J M,TUO L,HABDEN X,et al.Nesterenkonia populi sp.nov.,a novel actinobacterium isolated from Populus euphratica[J].Int J Syst Evol Microbiol,2015,65(5):1474-1479.
[16] 周双清,黄小龙,黄东益,等.Chelex-100快速提取放线菌DNA作为PCR扩增模板[J].生物技术通报,2010(2):123-125.
[17] ALTSCHUL S F,MADDEN T L,SCHAFFER A A,et al.Gapped BLAST and PSI-BLAST:a new generation of protein database search programs[J].Nucleic Acids Res,1997,25(17):3389-3402.
[18] KIM O S,CHO Y J,LEE K,et al.Introducing Ez-Taxone:a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species[J].Int J Syst Evol Microbiol,2012,62(3):716-721.
[19] KIMURA M.The neutral theory of molecular evolution[M].London:Cambridge University Press,1983:55-97.
[20] TAMURA K,PETERSON D,PETERSON N,et al.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Molecular Biology and Evolution,2011,28(10):2731-2739.
[21] SAITOU N,NEI M.The neighbour-joining method:a new method for reconstructing phylogenetic trees[J].Molecular Biology and Evolution,1987,4(4):406-425.
[22] FELSENTEIN J.Confidence limits on phylogenies:an approach using the bootstrap[J].Evolution,1985,39:783-791.
[23] MARMUR J,DOTY P.Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature[J].J Mol Biol,1962,5(1):109-118.
[24] DELEY J,CATTOIR H,REYNAERTS A.The quantita-tive measurement of DNA hybridization from renatura-tion rates[J].Eur J Biochem,1970,12(1):133-142.
[25] 马利,张利平,赵刚勇,等.放线菌的多相分类方法[J].河北省科学院学报,2004(1):46-51.
[26] CHUN J,RAINEY F A.Integrating genomics into the taxonomy and systematics of the bacteria and archaea[J].Int J Syst Evol Microbiol,2014,64(2):316-324.

备注/Memo

备注/Memo:
收稿日期:2018-06-12 录用日期:2018-11-23
基金项目:自治区重点研发计划(2016B03041-2); 新疆维吾尔自治区自然科学基金(2015211A038); 新疆干旱区植物逆境重点实验室项目(XJNUSYS112018A01)
*通信作者:xugela@163.com
更新日期/Last Update: 1900-01-01