|本期目录/Table of Contents|

[1]林燕燕,许新恒,黄仲庆,等.白凤菜总黄酮对肝癌HepG2细胞生长、增殖和凋亡的影响[J].厦门大学学报(自然科学版),2019,58(01):48-55.[doi:10.6043/j.issn.0438-0479.201807007]
 LIN Yanyan,XU Xinheng,HUANG Zhongqing,et al.Influence of total flavonoids of Gynura formosana Kitam.on the growth,proliferation and apoptosis of hepatoma HepG2 cells[J].Journal of Xiamen University(Natural Science),2019,58(01):48-55.[doi:10.6043/j.issn.0438-0479.201807007]
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白凤菜总黄酮对肝癌HepG2细胞生长、增殖和凋亡的影响(PDF/HTML)
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
58卷
期数:
2019年01期
页码:
48-55
栏目:
研究论文
出版日期:
2019-01-24

文章信息/Info

Title:
Influence of total flavonoids of Gynura formosana Kitam.on the growth,proliferation and apoptosis of hepatoma HepG2 cells
文章编号:
0438-0479(2019)01-0048-08
作者:
林燕燕1许新恒2黄仲庆1林泽燕1罗秀针1石 艳2*
1.漳州卫生职业学院基础医学部,福建 漳州 363000; 2.厦门大学生命科学学院,福建 厦门 361102
Author(s):
LIN Yanyan1XU Xinheng2HUANG Zhongqing1LIN Zeyan1LUO Xiuzhen1SHI Yan2*
1.Faculty of Basic Medicine,Zhangzhou Health Vocational College,Zhangzhou 363000,China; 2.School of Life Sciences,Xiamen University,Xiamen 361102,China
关键词:
白凤菜 总黄酮 细胞增殖 细胞周期 细胞凋亡
Keywords:
Gynura formosana Kitam. total flavonoids cell proliferation cell cycle cell apoptosis
分类号:
R 915
DOI:
10.6043/j.issn.0438-0479.201807007
文献标志码:
A
摘要:
研究了白凤菜(Gynura formosana Kitam.)总黄酮(TFG)对肝癌HepG2细胞的生长、增殖和凋亡的影响.噻唑兰(MTT)实验结果表明TFG对HepG2细胞体外增殖的抑制作用有浓度和时间依赖效应:处理24 h后130和260 μg/mL TFG实验组的HepG2细胞凋亡率均显著升高,分别达到(47.00±1.31)%和(76.39±1.39)%(p<0.05); 24 h的半抑制质量浓度(IC50)为190.80 μg/mL.实验组HepG2细胞周期阻滞在S期,细胞迁移率随TFG质量浓度的升高而下降,细胞内活性氧(ROS)水平最终显著下降至对照组的6.9%(p<0.05),细胞中Bax表达量略微上调而Bcl-2表达量明显下调.综上,TFG抑制肝癌HepG2细胞增殖和诱导HepG2细胞凋亡的机制可能与下调细胞ROS水平、重构细胞内还原体系、上调促凋亡蛋白Bax以及下调凋亡抑制蛋白Bcl-2表达相关.
Abstract:
This paper investigated the effects of total flavonoids of Gynura formosana Kitam.(TFG)on the growth,proliferation and apoptosis of hepatoma HepG2 cells.The MTT assay detected that TFG had dose- and time-dependent effects on the proliferation inhibition of HepG2 cells.The apoptosis rates of HepG2 cells treated with TFG of 130 μg/mL and 260 μg/mL increased significantly to(47.00±1.31)% and(76.39±1.39)%,respectively(p<0.05),and the half-concentration of inhibition(IC50)in 24 h was 190.80 μg/mL.In the TFG-treated groups, the HepG2 cell cycle was remarkably retarded and stayed in S phase and the cell mobility decreased with the increasing TFG concentration; the reactive oxygen species(ROS)levels of the HepG2 cells finally declined to 6.9%(p<0.05); the Bax expression in cells was slightly up-regulated and Bcl-2 expression was obviously down-regulated.Taking together,the mechanism inhibiting the proliferation and inducing the apoptosis of HepG2 cells may be related to the reduction in the ROS levels,the rebuilding of the intracellular redox system,the up-regulation of apoptosis promoting protein Bax and down-regulation of apoptosis inhibiting protein Bcl-2.

参考文献/References:

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[24] FAN J,LI R,ZHANG R.Effect of Bcl-2 and Bax on survival of side population cells from hepatocellular carcinoma cells[J].World J Gastroeneerol,2007,13(45):6053-6059.[1] JEMAL A,BRAY F,CENTER M M,et al.Global cancer statistics[J].CA Cancer J Clin,2011,61(2):69-90.
[2] 叶胜龙.关注原发性肝癌多学科治疗[J].中国医学前沿杂志,2014,6(5):1-3.
[3] 吴立军.天然药物化学[M].北京:人民卫生出版社,2003:173-216.
[4] 林瑶,谭冰,韦文荣,等.不同提取法对广金钱草总黄酮抗氧化活性的影响研究[J].右江名族医学院学报,2016,38(4):364-367.
[5] 任丽平,李先佳,朱宝安.马鞭草总黄酮对HepG-2细胞增殖及侵袭力影响[J].中国公共卫生,2016,32(7):935-937.
[6] 林燕燕,罗秀针,曹林枝,等.超声下白凤菜总黄酮的优化提取及其体外抑菌活性研究[J].右江名族医学院学报,2017,39(2):90-93.
[7] 李先佳,任丽平,金少举.马鞭草总黄酮对肝癌HepG-2细胞凋亡的影响机制研究[J].现代预防医学,2017,44(15):2813-2816,2822.
[8] 赛米热·艾尼瓦尔热,木塔力甫·艾买提,古扎力努尔·买提沙地克,等.高良姜总黄酮对宫颈癌SiHa 细胞增殖和凋亡的影响[J].新疆医学大学学报,2015,38(8):958-962,967.
[9] 张艳霞.黄芩黄酮类化合物体外抗宫颈癌作用的研究[D].秦皇岛:燕山大学,2013:15.
[10] 邱年永.原色台湾药用植物图鉴:第四册[M].台北:南天书局,1986:244.
[11] SAHDEO P,SUBASH C,AMIT K.Reactive oxygen species(ROS)and cancer: role of antioxidative nutraceuticals[J].Cancer Letters,2017,387:95-105.
[12] 木合布力·阿布力孜,王永波,徐方野,等.异甘草素和3-甲氧基异甘草素对人肝癌Bel-7402细胞的抗癌活性[J].中国药理学通报,2014,30(6):887-888.
[13] 顾员印,丁淑梅,孙翠翠,等.白凤菜对机体糖耐量影响的研究[J].中国畜牧兽医,2017,44(5):1547-1551.
[14] MA J F,GUO C,PAN Y L,et al.Antioxidant and anti-inflammatory activities of ethyl acetate extract of Gynura formosana(Kitam.)leaves[J].Experiment and Thera-peutic Medicine,2017,14:2303-2309.
[15] 陈灿滨,陈志亮,薛钰,等.白凤菜醇提物对斑马鱼酒精性脂肪肝损伤的修复[J].牡丹江师范学院学报,2016(94):53-56.
[16] WAN Y,XU W,LIU L Y,et al.Therapeutical effect of Gynura formosana alcohol extract on nonalcoholic fatty liver disease in rats[J].New Century Science Press,2014,2(2):1-6.
[17] MA J F,WEI P F,GUO C,et al.The ethyl acetate extract of Gynura formosana Kitam.leaves inhibited cervical cancer cell proliferation via induction of autophagy[J].BioMed Research International,2018,2018:4780612.
[18] JENNIFER N,THOMAS G.ROS signalling in the biology of cancer[J].Cell & Developmental Biology,2018,80:50-64.
[19] SUN Z L,DONG J L,WU J.Juglanin induces apoptosis and autophagy in human breast cancer progression via ROS/JNK promotion[J].Biomedicine & Pharmacotherapy,2017,85:303-312.
[20] HADDAD J J.The role of Bax/Bcl-2 and pro-caspase peptides in hypoxia/reperfusion-dependent regulation of MAPK(ERK): discordant pro-teomic effect of MAPK(p38)[J].Protein Pept Lett,2007,14(4):361-371.
[21] LIANG M,LI A,LOU A,et al.Advanced oxidation protein products promote NADPH oxidase-dependent β-cell destruction and dysfunction through the Bcl-2/Bax apoptotic pathway[J].Lab Invest,2017,97(7):792-805.
[22] 李立祥,熊奇如,耿小平,等.肝细胞肝癌中5-LOX的表达及其与Bcl-2和Bax表达的关系[J].肝胆外科杂志,2008,16(1):24-27.
[23] FRANK E.BCL-2 proteins and apoptosis:recent insights and unknowns[J].Biochemical and Biophysical Research Communications,2018,500:26-34.
[24] FAN J,LI R,ZHANG R.Effect of Bcl-2 and Bax on survival of side population cells from hepatocellular carcinoma cells[J].World J Gastroeneerol,2007,13(45):6053-6059.

备注/Memo

备注/Memo:
收稿日期:2018-07-03 录用日期:2018-10-04
基金项目:国家自然科学基金(31571896); 福建省教育厅科技B类项目(JB14209); 漳州卫生职业学院自然科学项目(ZWYZ201807)
*通信作者:yshi@xmu.edu.cn
更新日期/Last Update: 1900-01-01