|本期目录/Table of Contents|

[1]徐秋颖,刘伟伟,王宝家,等.康复新液对转化生长因子-β1诱导人胚肺成纤维细胞MRC-5纤维化模型的干预作用[J].厦门大学学报(自然科学版),2018,57(03):432-437.[doi:10.6043/j.issn.0438-0479.201801006]
 XU Qiuying,LIU Weiwei,WANG Baojia,et al.Effect of Kangfuxin Liquid in Alleviating Human Fetal Lung Fibroblast MRC-5 Cell Fibrosis Model Induced by Transforming Growth Factor-β1[J].Journal of Xiamen University(Natural Science),2018,57(03):432-437.[doi:10.6043/j.issn.0438-0479.201801006]
点击复制

康复新液对转化生长因子-β1诱导人胚肺成纤维细胞MRC-5纤维化模型的干预作用(PDF/HTML)
分享到:

《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
57卷
期数:
2018年03期
页码:
432-437
栏目:
研究简报
出版日期:
2018-05-31

文章信息/Info

Title:
Effect of Kangfuxin Liquid in Alleviating Human Fetal Lung Fibroblast MRC-5 Cell Fibrosis Model Induced by Transforming Growth Factor-β1
文章编号:
0438-0479(2018)03-0432-06
作者:
徐秋颖1刘伟伟1王宝家1马秀英2高永翔1*
1.成都中医药大学基础医学院,四川 成都 610075; 2.药用美洲大蠊四川省重点实验室,四川 成都 610000
Author(s):
XU Qiuying1LIU Weiwei1WANG Baojia1MA Xiuying2GAO Yongxiang1*
1.Basic Medical College,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China; 2.Medicinal Periplaneta americana Key Laboratory of Sichuan Province,Chengdu 610000,China
关键词:
康复新液 肺纤维化 转化生长因子-β1 MRC-5细胞
Keywords:
Kangfuxin liquid pulmonary fibrosis transforming growth factor-β1 MRC-5 cell
分类号:
R 33
DOI:
10.6043/j.issn.0438-0479.201801006
文献标志码:
A
摘要:
为探讨康复新液对人胚肺成纤维细胞纤维化模型的干预作用,以转化生长因子(transforming growth factor,TGF)-β1诱导人胚肺成纤维细胞MRC-5构建细胞纤维化模型,并运用CCK-8(cell counting kit-8)法检测康复新液对MRC-5细胞的毒性.以测得的最高不致死给药浓度(80倍稀释)的康复新液干预TGF-β1诱导的MRC-5细胞模型,进而通过实时荧光定量PCR(qRT-PCR)和蛋白质免疫印迹(WB)检测细胞纤维化指标的mRNA及蛋白质表达水平变化.结果显示:10 ng/mL TGF-β1可成功诱导MRC-5细胞发生纤维化,且细胞内α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)、层黏连蛋白(laminin,LN)、纤维连结蛋白(fibronectin,FN)、结缔组织生长因子(connective tissue growth factor,CTGF)、Ⅰ型胶原蛋白(CollagenⅠ)和Ⅲ型胶原蛋白(CollagenⅢ)的mRNA表达水平均显著升高; 以80倍稀释的康复新液为最高不致死给药浓度,对MRC-5细胞纤维化模型进行干预,上述纤维化指标的mRNA及蛋白质表达水平均出现显著下降,与地塞米松(Dexamethasone,DXM)的干预效果相当.综上,康复新液可改善由TGF-β1诱导MRC-5细胞纤维化的表型,有望被应用于肺纤维化的治疗.
Abstract:
To investigate the effect of Kangfuxin liquid on the pulmonary fibrosis cell model,the pulmonary fibrosis cell model was established by treating human fetal lung fibroblast MRC-5 cell with transforming growth factor-β1(TGF-β1),and the toxic effect of Kangfuxin liquid on MRC-5 cell was detected by cell-counting kit-8(CCK-8)assay.Then,Kangfuxin liquid diluted by 80 times was implemented on MRC-5 cell model induced by TGF-β1,and the mRNA and protein expression levels of fibrosis indexes were detected by qRT-PCR and Western blotting.The results showed that the pulmonary fibrosis cell model was established by treating human fetal lung fibroblast MRC-5 cell with TGF-β1 at the dose of 10 ng/mL.Kangfuxin liquid could significantly alleviate pulmonary fibrosis degree and efficiently down-regulate expression levels of α-SMA,LN,FN,CTGF,Collagen Ⅰ and Collagen Ⅲ in the pulmonary fibrosis cell model,with the comparable effect with Dexamethasone.Thus Kangfuxin liquid could significantly alleviate pulmonary fibrosis degree and might be a potential anti-fibrosis drug.

参考文献/References:

[1] 徐慧蓉,马小兵,王献华.肺纤维化相关因子研究进展[J].现代预防医学,2011,38(10):1953-1955.
[2] 李奇娟,王战国,刘巧,等.美洲大蠊研究现状及其研究中关键问题分析与展望[J/OL].中国中药杂志,2018.[2018-02-10].https:∥www.cnki.net/kcms/doi/10.19540/j.cnki.cjcmm.20180201.009.
[3] 陈新,王洪,张艳萍.康复新液的最新临床应用[J].中国医药指南,2016,6(17):89-96.
[4] THIERY J P,ACLOQUE H,HUANG R Y,et al.Epithelial-mesenchymal transitions in development and disease[J].Cell,2009,139(5):871-890.
[5] RADISKY D C,LEVY D D,LITTLEPAGE L E,et al.Rac1b and reactive oxygen species mediate MMP-3-induced EMT and genomic instability[J].Nature,2005,436(47):123-127.
[6] 刘森炎,钱一欣,卞蓉蓉,等.结缔组织生长因子通过上调肾间质成纤维细胞组织型转谷氨酰胺酶和Ⅲ型胶原蛋白促进肾间质纤维化[J].上海医学,2017,40(3):173-178.
[7] 滕立霞,于俊生.升降散对系膜增生性肾小球肾炎大鼠肾组织CTGF及α-SMA表达的影响[J].中华中医药学刊,2011,29(6):1358-1360.
[8] 陈春宇,陈志强.化瘀通络中药对糖尿病肾病大鼠肾组织纤维连接蛋白和层粘连蛋白的影响[J].天然产物研究与开发,2015,27:2134-2137.
[9] 郭治,吴金香.IL-33/ST2激活人肺成纤维细胞表达纤维黏连蛋白Ⅰ和Ⅰ型胶原蛋白参与哮喘气道重塑[J].细胞与分子免疫学杂志,2014,30(9):975-979.
[10] 朱锦东.牛磺熊去氧胆酸缓解转化生长因子-β1诱导的MRC-5纤维化表型[D].北京:北京协和医学院,2015:12-23.
[11] 潘有禄,黄文海,沈正荣.肺纤维化发生机制及治疗研究进展[J].中国药学杂志,2012,47(23):1873-1876.
[12] 周艳.基于“疏通肺络法”研究艾灸对肺纤维化大鼠上皮细胞-间质细胞转分化机制的影响[D].成都:成都中医药大学,2015:1.
[13] 陈茹萱.中药红景天成分对TGF-β1诱导MRC-5细胞纤维化表型的干预 [D].北京:北京协和医学院,2016:8.
[14] 程飚,付小兵,盛志勇,等.瘢痕组织中α-平滑肌肌动蛋白的表达与细胞凋亡的关系[J].中国病理生理杂志,2002,18(11):1333-1336.

备注/Memo

备注/Memo:
收稿日期:2018-01-04 录用日期:2018-03-05
基金项目:成都市科技惠民项目(2015-HM01-00466-SF)
*通信作者:yxgaocd@qq.com
引文格式:徐秋颖,刘伟伟,王宝家,等.康复新液对转化生长因子-β1诱导人胚肺成纤维细胞MRC-5纤维化模型的干预作用[J].厦门大学学报(自然科学版),2018,57(3):432-437.
Citation:XU Q Y,LIU W W,WANG B J,et al.Effect of Kangfuxin liquid in alleviating human fetal lung fibroblast MRC-5 cell fibrosis model induced by transforming growth factor-β1[J].J Xiamen Univ Nat Sci,2018,57(3):432-437.(in Chinese)
更新日期/Last Update: 1900-01-01