|本期目录/Table of Contents|

[1]刘子明,王桂忠*,李少菁,等.低温季节不同种群拟穴青蟹线粒体呼吸速率和酶活性的差异[J].厦门大学学报(自然科学版),2018,57(03):354-362.[doi:10.6043/j.issn.0438-0479.201710004]
 LIU Ziming,WANG Guizhong*,LI Shaojing,et al.Mitochondrial Respiration Rate and Enzyme Activity of Two Populations of Scylla paramamosain During Low Temperature Seasons[J].Journal of Xiamen University(Natural Science),2018,57(03):354-362.[doi:10.6043/j.issn.0438-0479.201710004]
点击复制

低温季节不同种群拟穴青蟹线粒体呼吸速率和酶活性的差异(PDF/HTML)
分享到:

《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
57卷
期数:
2018年03期
页码:
354-362
栏目:
研究论文
出版日期:
2018-05-31

文章信息/Info

Title:
Mitochondrial Respiration Rate and Enzyme Activity of Two Populations of Scylla paramamosain During Low Temperature Seasons
文章编号:
0438-0479(2018)03-0354-09
作者:
刘子明王桂忠*李少菁陈志刚
厦门大学 海洋与地球学院,近海海洋环境科学国家重点实验室,福建 厦门 361102
Author(s):
LIU ZimingWANG Guizhong*LI ShaojingCHEN Zhigang
State Key Laboratory of Marine Environmental Science,College of Ocean and Earth Sciences,Xiamen University,Xiamen 361102,China
关键词:
拟穴青蟹 种群 线粒体呼吸速率 酶活性 纬度差异
Keywords:
Scylla paramamosain population mitochondrial respiration rate enzyme activity latitude difference
分类号:
P 735
DOI:
10.6043/j.issn.0438-0479.201710004
文献标志码:
A
摘要:
已有研究显示分布于我国东南沿海的拟穴青蟹(Scylla paramamosain)可能已分化为南、北2个种群.在低温季节从不同纬度的3个海域采集拟穴青蟹的野生个体,比较其南、北种群线粒体呼吸速率和酶活性的差异.结果如下:除了乳酸脱氢酶、丙酮酸激酶以及肝胰腺细胞色素C氧化酶外,无论是北方种群还是南方种群,生活在纬度较高的宁波海域的拟穴青蟹的线粒体呼吸速率和酶活性都显著高于生活在纬度较低的儋州海域的个体; 同一海域的南、北种群间,鳃、肌肉和肝胰腺线粒体呼吸速率和细胞色素C氧化酶活性总体上都是北方种群高于南方种群,纬度越高的海域的南、北种群间差异越显著; 南、北种群肌肉的乳酸脱氢酶、丙酮酸激酶和己糖激酶活性相比,宁波海域北方种群的己糖激酶活性显著高于该海域的南方种群.上述结果表明,分布于我国东南沿海的拟穴青蟹的北方种群比南方种群对温度有更有效的代谢补偿能力,且纬度越高,二者的差异越显著,由此可见分布于我国东南沿海的拟穴青蟹的北方种群比南方种群更能适应低温环境.
Abstract:
Recent research has identified that the mud crab,Scylla paramamosain,in the southeast coast of China can be sub-divi-ded into northern and southern populations.The present study measured and compared the mitochondrial respiration rates and enzyme activities of northern and southern populations of the mud crab,S. paramamosain,sampled during low temperature seasons and from three locations at different latitudes.It was shown that for both northern and southern populations,with the exception of lactate dehydrogenase,pyruvate kinase and cytochrome C oxidase in hepatopancreas,the mitochondrial respiration rates and enzyme activities of crabs collected from higher latitude Ningbo coastal waters were significantly higher than those from Danzhou coastal waters at lower latitude.As for northern and southern populations collected at the same location,the mitochondrial respiration rates and cytochrome C oxidase activities in the gill,muscle and hepatopancreas of crabs of the northern population were generally higher than those of the southern population from all three locations with the differences increasing with latitude.For lactate dehydrogenase,pyruvate kinase and hexokinase activities in muscle,hexokinase activities of crabs of the northern population were also significantly higher than those of the southern population collected from Ningbo coastal waters.The results of the present study indicated that the northern population of S. paramamosain had strong metabolic compensation capacity than the southern population under cold environments,especially at higher latitude,confirming the hypothesis that the northern population of S. paramamosain adapted better to low temperature than the southern population.

参考文献/References:

[1] KEENAN C P,DAVIE P J F,MANN D L.A revision of the genus Scylla de Hann,1833(Crustacea:Decapoda:Brachyura:Portunidae)[J].Raffles Bulletin of Zoology,1998,46:217-245.
[2] 黎中宝,李少菁,王桂忠.中国东南沿海锯缘青蟹群体的形态判别分析[J].厦门大学学报(自然科学版),2004,43(1):101-106.
[3] 黎中宝,李少菁,王桂忠,等.锯缘青蟹等位酶的生化遗传分析[J].中国生态农业学报,2004,12(2):61-64.
[4] LI Z B,LI S J,WANG G Z.Genetic diversity and differentiation of mud crab Scylla serrate[J].Acta Oceanologica Sinica,2004,23(2):309-316.
[5] 马凌波,张凤英,乔振国,等.中国东南沿海青蟹线粒体CO1基因部分序列分析[J].水产学报,2006,30(4):463-468.
[6] 高天翔,王玉江,刘进贤,等.基于线粒体12S rRNA序列探讨4种青蟹系统发育关系及中国沿海青蟹的分类地位[J].中国海洋大学学报,2007,37(1):57-60.
[7] 林琪,李少菁,黎中宝,等.中国东南沿海青蟹属(Scylla)的种类组成[J].水产学报,2007,31(2):211-219.
[8] 林琪,李少菁,黎中宝,等.中国东南沿海青蟹属不同种类的mtDNA CO1基因序列分析及其系统发育[J].厦门大学学报(自然科学版),2008,47(2):268-273.
[9] 李少菁,王桂忠.锯缘青蟹生物学及人工育苗和养成技术[M].厦门:厦门大学出版社,2007:806.
[10] 王桂忠,李少菁,陈志刚.青蟹(Scylla spp.)养殖现状及拟穴青蟹(S. paramamosain)种群生物学研究[J].厦门大学学报(自然科学版),2016,55(5):617-623.
[11] 许晓军.拟穴青蟹(Scylla paramamosain)微卫星标记的开发和应用[D].厦门:厦门大学,2009:118.
[12] LIU Z M,WANG G Z,WU L S,et al.Seasonal change in mitochondrial function and metabolic enzyme activity of different populations of the mud crab,Scylla paramamosain,from China[J].Aquaculture,2012,376/377/378/379:68-75.
[13] SMITH P K,KROHN R I,HERMANSON G T,et al.Measurement of protein using bicinchoninic acid[J].A-nalytical Biochemistry,1985,150(1):176-185.
[14] KRAFFE E,MARTY Y,GUDERLEY H.Changes in mitochondrial oxidative capacities during thermal acclimation of rainbow trout Oncorhynchus mykiss:roles of membrane proteins,phospholipids and their fatty acid compositions[J].The Journal of Experimental Biology,2006,210:149-165.
[15] HULBERT A J,TURNER N,HINDE J,et al.How might you compare mitochondria from different tissues and different species?[J].Journal of Comparative Phy-siology B,2006,176:93-105.
[16] DUERR J M,PODRABSKY J E.Mitochondrial physio-logy of diapausing and developing embryos of the annual killifish Austrofundulus limnaeus:implications for extreme anoxia tolerance[J].Journal of Comparative Phy-siology B,2010,180(7):991-1003.
[17] THIBAULT M,BLIER P U,GUDERLEY H.Seasonal variation of muscle metabolic organization in rainbow trout(Oncorhynchus mykiss)[J].Fish Physiology and Biochemistry,1997,16(2):139-155.
[18] HANSEN J I,MUSTAFA T,DEPLEDGE M.Mechanisms of copper toxicity in the shore crab,Carcinus maenas. Ⅱ.Effects on key metabolic enzymes,metabolites and energy charge potential[J].Marine Bio-logy,1992,114(2):259-264.
[19] 李孝聪.中国区域历史地理[M].北京:北京大学出版社,2004:258-302.
[20] WANG G Z,KONG X H,WANG K J,et al.Variation of specific proteins,mitochondria and fatty acid composition in gill of Scylla serrata(Crustacea,Decapoda)under low temperature adaptation[J].Journal of Experimental Marine Biology and Ecology,2007,352(1):129-138.
[21] KONG X H,WANG G Z,LI S J.Seasonal variations of ATPase activity and antioxidant defenses in gills of the mud crab Scylla serrata(Crustacea,Decapoda)[J].Marine Biology,2008,154(2):269-276.
[22] LESSER M P,KRUSE V A.Seasonal temperature compensation in the horse mussel,Modiolus modiolus:me-tabolic enzymes,oxidative stress and heat shock proteins[J].Comparative Biochemistry and Physiology A,2004,137(3):495-504.
[23] BERNER N J,BESSAY E P.Correlation of seasonal acclimatization in metabolic enzyme activity with preferred body temperature in the Eastern red spotted newt(Notophthalmus viridescens viridescens)[J].Comparative Biochemistry and Physiology A,2006,144(4):429-436.
[24] YAN Y L,XIE X J.Liver mitochondrial and whole-animal level metabolic compensation in a catfish during seasonal acclimatization[J].Current Zoology,2011,57(1):109-115.
[25] DHILLON R S,SCHULTE P M.Intraspecific variation in the thermal plasticity of mitochondria in killifish[J].The Journal of Experimental Biology,2011,214(21):3639-3648.
[26] LUCASSEN M,KOSCHNICK N,ECKERLE L G,et al.Mitochondrial mechanisms of cold adaptation in cod(Gadus morhua L.)populations from different climatic zones[J].The Journal of Experimental Biology,2006,209(13):2462-2471.
[27] FANGUE,N A,RICHARDS J G,SCHULTE P M.Do mitochondrial properties explain intraspecific variation in thermal tolerance?[J].The Journal of Experimental Bio-logy,2009,212(4):514-522.

备注/Memo

备注/Memo:
收稿日期:2017-10-09 录用日期:2017-12-19
基金项目:国家自然科学基金(31472294)
*通信作者:gzwang@xmu.edu.cn
引文格式:刘子明,王桂忠,李少菁,等.低温季节不同种群拟穴青蟹线粒体呼吸速率和酶活性的差异[J].厦门大学学报(自然科学版),2018,57(3):354-362.
Citation:LIU Z M,WANG G Z,LI S J,et al.Mitochondrial respiration rate and enzyme activity of two populations of Scylla paramamosain during low temperature seasons[J].J Xiamen Univ Nat Sci,2018,57(3):354-362.(in Chinese)
更新日期/Last Update: 1900-01-01