|本期目录/Table of Contents|

[1]江 聪,尹大强,许鑫琦,等.齿毛菌漆酶对活性亮蓝的高效脱色[J].厦门大学学报(自然科学版),2018,57(03):337-345.[doi:10.6043/j.issn.0438-0479.201709024]
 JIANG Cong,YIN Daqiang,XU Xinqi,et al.Efficient Decolorization of Remazol Brilliant Blue Rby Cerrena unicolor Laccase[J].Journal of Xiamen University(Natural Science),2018,57(03):337-345.[doi:10.6043/j.issn.0438-0479.201709024]
点击复制

齿毛菌漆酶对活性亮蓝的高效脱色(PDF/HTML)
分享到:

《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
57卷
期数:
2018年03期
页码:
337-345
栏目:
研究论文
出版日期:
2018-05-31

文章信息/Info

Title:
Efficient Decolorization of Remazol Brilliant Blue Rby Cerrena unicolor Laccase
文章编号:
0438-0479(2018)03-0337-09
作者:
江 聪1尹大强2许鑫琦1林 娟1叶秀云1杨 捷1*
1.福州大学福建省海洋酶工程重点实验室,福建 福州 350116; 2.同济大学长江水环境教育部重点实验室,上海 200092
Author(s):
JIANG Cong1YIN Daqiang2XU Xinqi1LIN Juan1YE Xiuyun1YANG Jie1*
1.Fujian Key Laboratory of Marine Enzyme Engineering,Fuzhou University,Fuzhou 350116,China; 2.Key Laboratory of Yangtze River Water Environment,Ministry of Education,Tongji University,Shanghai 200092,China
关键词:
漆酶 活性亮蓝 脱色 响应面法 红外光谱 薄层色谱
Keywords:
laccase Remazol Brilliant Blue R decolorization response surface method infrared spectrometry thin layer chromatography
分类号:
Q 89
DOI:
10.6043/j.issn.0438-0479.201709024
文献标志码:
A
摘要:
漆酶是一种含铜的多酚氧化酶,在环境污染治理等领域具有广阔的应用前景.采用齿毛菌(Cerrena unicolor)漆酶对蒽醌染料活性亮蓝(Remazol Brilliant Blue R,RBBR)进行催化脱色.在单因素试验的基础上,应用中心组合设计(central composite design,CCD)建立4因素3水平的响应面法分析模型,得到齿毛菌漆酶对RBBR脱色的最佳条件:漆酶浓度2.24 U/mL,染料质量浓度123.83 mg/L,pH 4.52,脱色时间38.30 min,预测理论脱色率为99.82%.RBBR在最佳脱色条件下的实际脱色率为(98.42±1.39)%.影响脱色效果的因素主次顺序为:pH>酶浓度>脱色时间>染料质量浓度.红外光谱和薄层色谱分析发现齿毛菌漆酶处理后RBBR的结构发生改变,其中的C—N键发生断裂,证明该漆酶确实催化了RBBR的降解.齿毛菌漆酶对RBBR脱色反应的米氏常数Km值为(134.82 ±3.05)mg/L,不需要氧化还原介体就可以对RBBR进行高效脱色,在染料脱色和生物修复方面具有实际应用价值.
Abstract:
Laccases are members of a family of copper-containing polyphenol oxidases and have wide applications in bioremediation.In this study,catalytic decolorization of Remazol Brilliant Blue R(RBBR)by Cerrena unicolor laccase was investigated.Based on the single factor experiments,the central composite design(CCD)was used to establish the four-factor three-level response surface method analysis model,and the optimum conditions of laccase-mediated RBBR decolorization were as follows:2.24 U/mL laccase,123.83 mg/L RBBR,pH 4.52,and 38.30 min.The predicted and observed decolorization rates were 99.82% and(98.42±1.39)%,respectively,under the optimal conditions.Influences of the factors on the decolorization efficiency were pH> laccase concentration> decolorization time> dye concentration.The infrared spectrometry and thin layer chromatography analyses showed that the structure of RBBR changed due to the laccase treatment.Specifically,the C—N bond in RBBR was broken upon laccase treatment,corroborating that the laccase catalyzed RBBR degradation.The Km value of the C. unicolor laccase towards RBBR was(134.82±3.05)mg/L,as indicated by the kinetic study,and efficiently decolorized RBBR in the absence of a redox mediator,showing promise in dye decolorization and bioremediation.

参考文献/References:

[1] 纪立军.我国染料工业现状与发展趋势[J].新材料产业,2011,11:54-58.
[2] 曾祥康.毛栓菌发酵产漆酶及其在合成染料脱色中的应用[D].无锡:江南大学,2012:2-3.
[3] 李瑞云,栗君.漆酶对染料废水脱色的应用研究[J].环境科学与管理,2015,40(9):98-100.
[4] OSMA J F,TOCA-HERRERA J L,RODRíGUEZ-COUTO S.Transformation pathway of Remazol Brilliant Blue R by immobilised laccase[J].Bioresource Technology,2010,101(22):8509-8514.
[5] LU L,ZHAO M,ZHANG B B,et al.Purification and characterization of laccase from Pycnoporus sanguineus and decolorization of an anthraquinone dye by the enzyme[J].Applied Microbiology & Biotechnology,2006,74(6):1232.
[6] YANG J,LI W,NG T B,et al.Laccases:production,expression regulation,and applications in pharmaceutical biodegradation[J].Frontiers in Microbiology,2017,8:832.
[7] 武海龙.利用酶法-化学氧化法偶联工艺对活性艳蓝KN-R的脱色研究[D].福州:福建师范大学,2015:16.
[8] YANG J,LIN Q,NG T B,et al.Purification and characterization of a novel laccase from Cerrena sp. HYB07 with dye decolorizing ability[J].PLoS One,2014,9(10):e110834.
[9] AGHAIE-KHOUZANI M,FOROOTANFAR H,MOSHFEGH M,et al.Decolorization of some synthetic dyes using optimized culture broth of laccase producing ascomycete Paraconiothyrium variabile[J].Biochemical Engineering Journal,2012,60:9-15.
[10] 沈萍,陈向东.微生物学实验[M].4版.北京:高等教育出版社,2007:215.
[11] SATHISHKUMAR P,BALAN K,PALVANNAN T,et al.Efficiency of Pleurotus florida,laccase on decolorization and detoxification of the reactive dye Remazol Brilliant Blue R(RBBR)under optimized conditions[J].Clean Soil Air Water,2013,41(7):665-672.
[12] SOARES G M B,AMORIM M T P D,COSTA-FERREIRA M.Use of laccase together with redox mediators to decolourize Remazol Brilliant Blue R[J].Journal of Biotechnology,2001,89(2/3):123-129.
[13] MECHICHI T,MHIRI N,SAYADI S.Remazol Brilliant Blue R decolourization by the laccase from Trametes trogii[J].Chemosphere,2006,64(6):998-1005.
[14] KHLIFI R,SAYADI S,BELBAHRI L,et al.Effect of HBT on the stability of laccase during the decolourization of textile wastewaters[J].Journal of Chemical Technology & Biotechnology,2009,84(12):1828-1833.
[15] KUNAMNENI A,GHAZI I,CAMARERO S,et al.Decolorization of synthetic dyes by laccase immobilized on epoxy-activated carriers[J].Process Biochemistry,2008,43(2):169-178.
[16] MURUGESAN K,NAM I H,KIM Y M,et al.Decolorization of reactive dyes by a thermostable laccase produced by Ganoderma lucidum,in solid state culture[J].Enzyme & Microbial Technology,2007,40(7):1662-1672.
[17] 王天女,李国富,赵敏,等.重组血红密孔菌漆酶在染料脱色中的应用[J].南京林业大学学报(自然科学版),2016,40(1):92-96.
[18] HU M R,CHAO Y G,XUE Z Q,et al.Laccase-mediator system in the decolorization of different types of recalcitrant dyes[J].Journal of Industrial Microbiology & Biotechnology,2009,36(1):45-51.
[19] ADAK A,TIWARI R,SINGH S,et al.Laccase production by a novel white-rot fungus Pseudolagarobasidium acaciicola LA 1 through solid-state fermentation of parthenium,biomass and its application in dyes decolorization[J].Waste & Biomass Valorization,2016,7(6):1427-1435.
[20] LE T T,MURUGESAN K,LEE C S,et al.Degradation of synthetic pollutants in real wastewater using laccase encapsulated in core-shell magnetic copper alginate beads[J].Bioresource Technology,2016,216:203-210.
[21] 侯红漫,周集体,王竟,等.白腐菌糙皮侧尔漆酶性质及其对蒽醌染料脱色性能的研究[J].林产化学与工业,2004,24(1):48-52.
[22] 戴文魁.白腐菌混合培养产漆酶及其机制和脱色效果研究[D].济南:山东大学,2008:44.

备注/Memo

备注/Memo:
收稿日期:2017-09-20 录用日期:2018-01-17
基金项目:国家自然科学基金(31671795); 福建省引导性项目(2016Y0059); 海洋生物酶工程创新服务平台项目(2014FJPT02); 福州大学贵重仪器设备开放测试基金(2017T027)
*通信作者:T09136@fzu.edu.cn
引文格式:江聪,尹大强,许鑫琦,等.齿毛菌漆酶对活性亮蓝的高效脱色[J].厦门大学学报(自然科学版),2018,57(3):337-345.
Citation:JIANG C,YIN D Q,XU X Q,et al.Efficient decolorization of Remazol Brilliant Blue R by Cerrena unicolor laccase[J].J Xiamen Univ Nat Sci,2018,57(3):337-345.(in Chinese)
更新日期/Last Update: 1900-01-01