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[1]翁振宇,闵小平*,王 海,等.对流实时荧光定量PCR系统设计[J].厦门大学学报(自然科学版),2018,57(01):130-136.[doi:10.6043/j.issn.0438-0479.201705046]
 WENG Zhenyu,MIN Xiaoping*,WANG Hai,et al.Design on Real-time Fluorescence Quantitative PCR System Based on Natural Convection[J].Journal of Xiamen University(Natural Science),2018,57(01):130-136.[doi:10.6043/j.issn.0438-0479.201705046]
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
57卷
期数:
2018年01期
页码:
130-136
栏目:
研究论文
出版日期:
2018-01-26

文章信息/Info

Title:
Design on Real-time Fluorescence Quantitative PCR System Based on Natural Convection
文章编号:
0438-0479(2018)01-0130-07
作者:
翁振宇1闵小平23*王 海2卓之豪3葛胜祥3
1.厦门大学公共卫生学院,福建 厦门 361102; 2厦门大学信息科学与技术学院,福建 厦门 361005; 3.国家传染病诊断试剂与疫苗工程技术研究中心(厦门大学),福建 厦门 361102
Author(s):
WENG Zhenyu1MIN Xiaoping23*WANG Hai2ZHUO Zhihao3GE Shengxiang3
1.Schoolof Public Health,Xiamen University,Xiamen 361102,China; 2.School of Information Science and Engineering,Xiamen University,Xiamen 361005,China; 3.National Institute of Diagnostics and Vaccine Development in Infection Diseases(Xiamen University),X
关键词:
实时荧光定量PCR 自然对流PCR 双通道 快速扩增
Keywords:
real-time fluorescence quantitative PCR natural convection PCR two-channel structure rapid amplification
分类号:
TH 776
DOI:
10.6043/j.issn.0438-0479.201705046
文献标志码:
A
摘要:
设计了一种结合实时荧光定量技术和自然对流技术的PCR系统.主要介绍该系统的双通道荧光激发与检测结构及其工作原理,系统中使用光纤作为光路的传输媒介,采用电荷耦合元件(CCD)工业相机采集荧光信号变化,并自行设计电路和软件驱动整套系统正常运行.最后通过对梯度稀释的巨细胞病毒(CMV)的基因模板进行扩增,展示了该系统可用于快速扩增和实时定量检测的潜力.
Abstract:
A PCR system with real-time fluorescent quantitative technology and natural convection technology was designed.This paper mainly introduced the two-channels structure and the work principle of fluorescence excitation and fluorescence detection.In the system,fiber optics were used as an optical transmission medium and a charge coupled device(CCD)camera was applied to collect the change of fluorescent signal.To ensure that the system work steadily,the circuit and software were designed independently.Finally,we showed the system’s potential of rapid nucleic acid amplification and real-time quantitation by amplifying gradient-diluted gene template of cytomegalovirus(CMV).

参考文献/References:

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[6] 曹仙春.荧光定量PCR仪的设计[J].山西电子技术,2005(4):38-40.
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备注/Memo

备注/Memo:
收稿日期:2017-05-29 录用日期:2017-09-26
*通信作者:mxp@xmu.edu.cn
更新日期/Last Update: 1900-01-01