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[1]张瑞娟,柯莉娜,郑 静,等.鲍鱼内脏多糖的提取、纯化及其抗氧化和抑菌活性[J].厦门大学学报(自然科学版),2018,57(01):58-64.[doi:10.6043/j.issn.0438-0479.201702038]
 ZHANG Ruijuan,KE Lina,ZHENG Jing,et al.Extraction,Purification and Antioxidant and Antibacterial Activity Analysis of Polysaccharides from Abalone Viscera[J].Journal of Xiamen University(Natural Science),2018,57(01):58-64.[doi:10.6043/j.issn.0438-0479.201702038]
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鲍鱼内脏多糖的提取、纯化及其抗氧化和抑菌活性(PDF/HTML)
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
57卷
期数:
2018年01期
页码:
58-64
栏目:
研究论文
出版日期:
2018-01-26

文章信息/Info

Title:
Extraction,Purification and Antioxidant and Antibacterial Activity Analysis of Polysaccharides from Abalone Viscera
文章编号:
0438-0479(2018)01-0058-07
作者:
张瑞娟柯莉娜郑 静石 艳王 勤*
厦门大学生命科学学院,福建 厦门 361102
Author(s):
ZHANG RuijuanKE LinaZHENG JingSHI YanWANG Qin*
School of Life Sciences,Xiamen University,Xiamen 361102,China
关键词:
鲍鱼内脏 多糖 提取 纯化 抗氧化活性 抑菌活性
Keywords:
abalone viscera polysaccharide extraction purification antioxidant activity antibacterial activity
分类号:
Q 936
DOI:
10.6043/j.issn.0438-0479.201702038
文献标志码:
A
摘要:
以皱纹盘鲍(Haliotis discus hannai Ino)内脏为实验材料,采用碱性蛋白酶、中性蛋白酶、酸性蛋白酶、胃蛋白酶、胰蛋白酶和木瓜蛋白酶6种蛋白酶对其进行酶解提取多糖,结果显示碱性蛋白酶酶解所得多糖得率最高,为6.66%,进而由单因素试验和正交试验确定了碱性蛋白酶提取鲍鱼内脏多糖的最佳工艺条件.将得到的鲍鱼内脏粗多糖进行体外抗氧化活性测定,结果显示:100%清除羟自由基、100%清除2,2-联氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二氨盐(ABTS)自由基以及700 nm处吸光度(A700)为0.485时代表的还原能力对应的鲍鱼内脏粗多糖质量浓度分别为9.6,7.5,8.2 mg/mL,表明所得粗多糖具有较好的抗氧化活性.采用双酶解法以提高多糖含量,结果显示胃蛋白酶二次酶解所得的多糖含量最高,且多糖样品在3种抗氧化活性模型中均具有很好的效果.采用Sevage法和反复冻融法去除蛋白,过氧化氢脱除色素,进一步纯化得到高纯度的鲍鱼内脏多糖,并采用双层平板法测定其抑菌活性,结果表明其对金黄色葡萄球菌(Staphyloccocus aureus)、鼠伤寒沙门氏菌(Salmonella typhimurium)、大肠杆菌(Escherichia coil)和铜绿假单胞菌(Pseudomonas aeruginosa)都具有较好的抑制作用.
Abstract:
The polysaccharides were obtained using abalone(Haliotis discus hannai Ino)viscera as the material in this study and enzymolyzed by alkaline protease,neutral protease,vernase,papain,pepsin and trypsin.The results of antioxidant experiments showed that the yield of polysaccharides was the highest up to 6.66% when alkaline protease was used.Then through the single factor experiment and the orthogonal experiment,the optimal condition for alkaline protease extracting polysaccharides from abalone visceral was determined.Results of antioxidant activity showed that the concentration of primary polysaccharides from abalone viscera for 100% removal of hydroxyl free radical was 9.6 mg/mL, the concentration for 100% removal of ABTS radical was 7.5 mg/mL, and the concentration of reducing capacity at A700=0.485 was 8.2 mg/mL. The results indicated that the polysaccharides had a good antioxidant activity.Additionally,the polysaccharide content and antioxidant activity were the highest with secondary enzymolyzing by pepsin.Furthermore,we got the high-purity abalone visceral polysaccharides with Sevage method and repeated freezing and thawing method to remove proteins, and with hydrogen peroxide method for depigment.Finally,double plate method was used to measure antibacterial activity of the abalone visceral polysaccharides,which showed that the purified polysaccharides suppressed reproduction of Staphylococcus aureus,Salmonella typhimurium,Escherichia coli and Pseudomonas aeruginosa efficently.The polysaccharides were obtained using abalone(Haliotis discus hannai Ino)viscera as the material in this study and enzymolyzed by alkaline protease,neutral protease,vernase,papain,pepsin and trypsin.The results of antioxidant experiments showed that the yield of polysaccharides was the highest up to 6.66% when alkaline protease was used.Then through the single factor experiment and the orthogonal experiment,the optimal condition for alkaline protease extracting polysaccharides from abalone visceral was determined.Results of antioxidant activity showed that the concentration of primary polysaccharides from abalone viscera for 100% removal of hydroxyl free radical was 9.6 mg/mL, the concentration for 100% removal of ABTS radical was 7.5 mg/mL, and the concentration of reducing capacity at A700=0.485 was 8.2 mg/mL. The results indicated that the polysaccharides had a good antioxidant activity.Additionally,the polysaccharide content and antioxidant activity were the highest with secondary enzymolyzing by pepsin.Furthermore,we got the high-purity abalone visceral polysaccharides with Sevage method and repeated freezing and thawing method to remove proteins, and with hydrogen peroxide method for depigment.Finally,double plate method was used to measure antibacterial activity of the abalone visceral polysaccharides,which showed that the purified polysaccharides suppressed reproduction of Staphylococcus aureus,Salmonella typhimurium,Escherichia coli and Pseudomonas aeruginosa efficently.

参考文献/References:

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[3] BECKER G L.Preserving food and health:antioxidants make functional,nutritious preservatives[J].Food Processing,1993(12):54-56.
[4] CHEN H X,ZHANG M,QU Z S,et al.Antioxidant acti-vities of different fractions of polysaccharide conjugates from green tea(Camellia sinensis)[J].Food Chemistry,2008,106(2):559-563.
[5] CHEN Y,XIE M Y,NIE S P,et al.Purification,composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum[J].Food Chemistry,2008,107(1):231-241.
[6] 罗晓航,余鑫,卢晓燕,等.鲍鱼脏器粗多糖体外抗氧化活性研究[J].中国海洋药物杂志,2012,31(6):10-16.
[7] 王莅莎,朱蓓薇,周大勇,等.鲍鱼脏器多糖的抗氧化活性研究[J].食品与机械,2009,24(4):65-68.
[8] 苏永昌,刘淑集,王茵,等.鲍鱼内脏多糖的提取及其抗氧化活性研究[J].吉林农业,2010(10):170-171.
[9] 朱莉莉,孙黎明,李冬梅,等.鲍鱼内脏蛋白多糖体内对H22肝癌的抑制作用[J].营养学报,2009,31(5):478-481.
[10] 王莅莎,朱蓓薇,孙黎明,等.鲍鱼内脏多糖的体外抗肿瘤和免疫调节活性研究[J].大连工业大学学报,2008,27(4):289-293.
[11] ZHU B W,LI D M,ZHOU D Y,et al.Structural analysis and CCK-releasing activity of a sulfated polysaccharide from abalone(Haliotis discus hannai Ino)viscera[J].Food Chemistry,2011,125(4):1273-1278.
[12] 张永勤,王哲平,宋雨梅,等.还原糖测定方法的比较研究[J].食品工业科技,2010,31(6):321-323.
[13] 程迪,于洁,董丽.河南产牡丹皮中多糖含量的测定[J].安徽农业科学,2008,36(2):518.
[14] 宿玮.高品质海地瓜多糖制备工艺研究[D].青岛:中国海洋大学,2012:26.
[15] 贾彦明,闵伟红.海带多糖的分离纯化及体外抗氧化作用的研究[J].农产品加工学刊,2010(8):26-29.
[16] 苏晓雨,王振宇.红松子种皮提取物活性成分及抗氧化作用研究[J].林产化学与工业,2010,30(4):99-102.
[17] 刘春燕.鲍鱼内脏多糖的结构和活性研究[D].长春:东北师范大学,2011:9.
[18] 陈健,耿安静,徐晓飞.香菇多糖的过氧化氢脱色工艺研究[J].食品工业科技,2010,31(3):293-295.
[19] ZHU Y J,SONG K K,LI Z C,et al.Antityrosinase and antimicrobial activities of trans-cinnamaldehyde thiosemicarbazone[J].Journal of Agricultural and Food Chemistry,2009,57(12):5518-5523.
[20] WANG Z J,LUO D H.Antioxidant activities of different fractions of polysaccharide purified from Gynostemma pentaphyllum Makino[J].Carbohydrate Polymers,2007,68(1):54-58.
[21] TSENG Y H,YANG J H,MAU J L.Antioxidant properties of polysaccharides from Ganoderma tsugae[J].Food Chemistry,2008,107(2):732-738.
[22] LI X M,LI X L,ZHOU A G,et al.Evaluation of antioxidant activity of the polysaccharides extracted from Lycium barbarum fruits in vitro[J].European Polymer Journal,2007,43(2):448-497.

备注/Memo

备注/Memo:
收稿日期:2017-02-22 录用日期:2017-05-15
基金项目:国家海洋公益性行业科研专项(201405016)
*通信作者:qwang@xmu.edu.cn
更新日期/Last Update: 1900-01-01