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[1]廖芸英,熊晓鹏*.黏度法研究牛血清白蛋白的构象[J].厦门大学学报(自然科学版),2017,56(03):456-460.[doi:10.6043/j.issn.0438-0479.201606036]
 LIAO Yunying,XIONG Xiaopeng*.Conformation of Bovine Serum Albumin Studied with Viscometric Method[J].Journal of Xiamen University(Natural Science),2017,56(03):456-460.[doi:10.6043/j.issn.0438-0479.201606036]
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
56卷
期数:
2017年03期
页码:
456-460
栏目:
研究简报
出版日期:
2017-05-24

文章信息/Info

Title:
Conformation of Bovine Serum Albumin Studied with Viscometric Method
文章编号:
0438-0479(2017)03-0456-05
作者:
廖芸英熊晓鹏*
厦门大学材料学院,福建厦门361005
Author(s):
LIAO YunyingXIONG Xiaopeng*
College of Materials,Xiamen University,Xiamen 361005,China
关键词:
黏度法特性黏数牛血清白蛋白构象
Keywords:
viscometric methodintrinsic viscositybovine serum albumin(BSA)conformation
分类号:
O 631
DOI:
10.6043/j.issn.0438-0479.201606036
文献标志码:
A
摘要:
采用乌氏黏度计测定不同pH条件下牛血清白蛋白(BSA)的黏度,运用Wolf方程描述BSA的黏度变化行为.详细讨论了不同pH条件下BSA的特性黏数(intrinsic viscosity,[η])与其构象的关系,并分析了BSA比特性黏数(specific intrinsic viscosity,{η})的组成变化.研究结果表明,采用这种新型的黏度法研究球状蛋白质的构象或溶液性质是科学、可行的.
Abstract:
The viscosities of bovine serum albuminin (BSA) in different pH buffers were measured using Ubbelohde capillary viscometer,and the data were evaluated by Wolf equation to describe the composition dependences of BSA.The intrinsic viscosities ([η]) of BSA under different pH were correlated with its conformation,and the composition dependences of specific intrinsic viscosities ({η}) of BSA were carefully analyzed.Our results indicate that the new viscometric method can be applied in the study of conformation and solution properties of spherical protein.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-06-23 录用日期:2016-09-08
基金项目:国家自然科学基金(51273166)
*通信作者:xpxiong@xmu.edu.cn
引文格式:廖芸英,熊晓鹏.黏度法研究牛血清白蛋白的构象[J].厦门大学学报(自然科学版),2017,56(3):456-460.
Citation:LIAO Y Y,XIONG X P.Conformation of bovine serum albumin studied with viscometric method[J].J Xiamen Univ Nat Sci,2017,56(3):456-460.(in Chinese)
更新日期/Last Update: 1900-01-01