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 ZU’?IGA-NAVARRETE F,GóMEZ I,PE?A G,et al.A Tenebriomolitor GPI-anchored alkaline phosphatase is involved in binding of Bacillus thuringiensis Cry3Aa to brush border membrane vesicles[J].Peptides,2013,41:81-86.Cloning,Prokaryotic Expression and Functional Analysis of PxCR10-11 Domain in Plutella xylostella CadherinXU Lian1,LIU Bo2,PAN Zhizhen2,ZHU Yujing2,GAO Huanjuan1,CHEN Qingxi1* (1.School of Life Sciences,Xiamen University,Xiamen 361102,China; 2.Agricultural Bio-resources Research Institute,Fujian Academy of Agriculture Sciences,Fuzhou 350003,China)Abstract:Cadherin,formed by a number of characteristic cadherin repeats(CR)domains,is a chain-like membrane glycoprotein.It was widely reported that cadherin in insect midgut played an essential role in the insecticidal activitives of Bacillus thuringiensis(Bt)toxins.The CR domain near the cell membrane was documented as an interaction region with Bt toxins.Plutella xylostella cadherin PxCR was formed by 11 CR domains and a transmembrane domain.The 10th and 11th CR domains(PxCR10-11)in PxCR were regarded as a potential interaction region with Bt toxins.In this study,PxCR10-11 domain was cloned from Plutella xylostella midgut cDNA,and then a prokaryotic expression vector pGEX4t-PxCR10-11 was constructed and the fusion protein GST-PxCR10-11 was induced to express.Ligand blot indicated that PxCR10-11 could specially bind to Cry2Ab and the bioassay revealed that PxCR10-11 had synergistic effect on Cry2Ab against Plutella xylostella larvae.Furthermore,homologous modeling of PxCR10-11 was conducted and the glycosylation sites in PxCR10-11 were predicted.These results indicate that PxCR10-11 is involved in the processing of Cry2Ab against Plutella xylostella larvae,which lays the foundation for the interaction mechanism of PxCR10-11 with Cry2Ab toxins.