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[1]刘富川,薛 勇,刘 健,等.转录调控因子BglR对东方肉座菌纤维素酶表达的影响[J].厦门大学学报(自然科学版),2018,57(04):494-502.[doi:10.6043/j.issn.0438-0479.201803020]
 LIU Fuchuan,XUE Yong,LIU Jian,et al.Effect of the Transcriptional Regulation Factor BglR on Cellulase Expression in Trichoderma orientalis[J].Journal of Xiamen University(Natural Science),2018,57(04):494-502.[doi:10.6043/j.issn.0438-0479.201803020]
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转录调控因子BglR对东方肉座菌纤维素酶表达的影响(PDF)
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
57卷
期数:
2018年04期
页码:
494-502
栏目:
研究论文
出版日期:
2018-07-31

文章信息/Info

Title:
Effect of the Transcriptional Regulation Factor BglR on Cellulase Expression in Trichoderma orientalis
文章编号:
0438-0479(2018)04-0494-09
作者:
刘富川薛 勇刘 健甘礼惠龙敏南*
厦门大学能源学院,福建 厦门 361102
Author(s):
LIU FuchuanXUE YongLIU JianGAN LihuiLONG Minnan*
College of Energy,Xiamen University,Xiamen 361102,China
关键词:
东方肉座菌 bglr基因 转录调控因子 β-葡萄糖苷酶
Keywords:
Trichoderma orientalis bglr gene transcriptional regulation factor β-glucosidase
分类号:
Q 939.97
DOI:
10.6043/j.issn.0438-0479.201803020
文献标志码:
A
摘要:
东方肉座菌(Trichoderma orientalis)EU7-22菌株是一株高效分泌纤维素酶的丝状真菌,对生物质转化具有重要价值.采用交错式热不对称PCR(TAIL-PCR)方法克隆得到纤维素酶转录调控因子bglr基因及其上下游序列,并经同源交换获得bglr基因敲除的菌株EU7-22Δbglr.其生产的滤纸酶、外切葡聚糖酶、木聚糖酶活力和分泌蛋白最高值较对照菌株分别增加了39%,22%,16%和20%,而β-葡萄糖苷酶活力下降47%,β-葡萄糖苷酶基因bgl1的表达量显著降低.进一步分析发现BglR的缺失抑制了菌株EU7-22在以特定多糖/寡糖为碳源的培养基上的生长,且引起发酵液pH值的改变.上述结果证明BglR对β-葡萄糖苷酶起正调控作用,可为构建高效降解纤维素的工程菌株提供参考.
Abstract:
As an efficient fungi of secreting cellulase,Trichoderma orientalis EU7-22 has an important value in the degradation of biomass.The bglr gene as well as the upstream and downstream sequences were cloned in EU7-22 using thermal asymmetric interlaced PCR according to the conserved sequence.Compared to the parent strain,the Δbglr knockout mutant constructed by homologous recombination showed that the enzyme activities(FPase,exoglucanase,xylanase)and the highest extracellular protein concentration increased by 39%,22%,16% and 20%,respectively,while the activity of β-glucosidase reduced by 47%,and the expression of the corresponding gene bgl1 also decreased.Moreover,The deletion of BglR inhibited the growth of the strain on a medium with the specific polysaccharide/oligosaccharide as a carbon source,and the pH value of the fermented supernatant also changed.Based on the results,it is proposed that BglR is a transcriptional factor for positive-regulating the β-glucosidase in EU7-22,which is expected in be applicable to further genetical modification for constructing high efficient cellulase-degradating microbe.

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备注/Memo

备注/Memo:
收稿日期:2018-03-07 录用日期:2018-04-09
基金项目:福建省海洋高新产业发展专项(闽海洋高新[2015]27号); 厦门大学能源发展基金(2017NYFZ02)
*通信作者:longmn@xmu.edu.cn
引文格式:刘富川,薛勇,刘健,等.转录调控因子BglR对东方肉座菌纤维素酶表达的影响[J].厦门大学学报(自然科学版),2018,57(4):494-502.
Citation:LIU F C,XUE Y,LIU J,et al.Effect of the transcriptional regulation factor BglR on cellulase expression in Trichoderma orientalis[J].J Xiamen Univ Nat Sci,2018,57(4):494-502.(in Chinese)
更新日期/Last Update: 1900-01-01