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[1]陈 姗,张芳芳,黄 贝,等.日本鳗鲡γ-干扰素诱导的溶酶体巯基还原酶(GILT)基因的克隆鉴定与表达分析[J].厦门大学学报(自然科学版),2018,57(04):484-493.[doi:doi:10.6043/j.issn.0438-0479.201803031]
 CHEN Shan,ZHANG Fangfang,HUANG Bei,et al.Cloning and Expression Analysis of Interferon-γ-inducible Lysosomal Thiol Reductase(GILT)Gene in Anguilla japonica[J].Journal of Xiamen University(Natural Science),2018,57(04):484-493.[doi:doi:10.6043/j.issn.0438-0479.201803031]
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日本鳗鲡γ-干扰素诱导的溶酶体巯基还原酶(GILT)基因的克隆鉴定与表达分析(PDF)
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
57卷
期数:
2018年04期
页码:
484-493
栏目:
研究论文
出版日期:
2018-07-31

文章信息/Info

Title:
Cloning and Expression Analysis of Interferon-γ-inducible Lysosomal Thiol Reductase(GILT)Gene in Anguilla japonica
文章编号:
0438-0479(2018)04-0484-10
作者:
陈 姗12张芳芳12黄 贝12黄文树123*
1.集美大学水产学院,2.鳗鲡现代产业技术教育部工程研究中心(集美大学), 3.福建省海洋生物资源开发利用协同创新中心,福建 厦门 361021
Author(s):
CHEN Shan 12ZHANG Fangfang 12HUANG Bei12HUANG Wenshu123*
1.Fishery College,Jimei University,2.Engineering Research Center of the Modern Technology for Eel Industry,Ministry of Education,Jimei University,3.Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources,Xiamen 361021,China
关键词:
日本鳗鲡 γ-干扰素诱导的溶酶体巯基还原酶 基因表达
Keywords:
Anguilla japonica interferon-γ interferon-ducible lysosomal thiol reductase gene expression
分类号:
Q 75
DOI:
doi:10.6043/j.issn.0438-0479.201803031
文献标志码:
A
摘要:
在日本鳗鲡(Anguilla japonica)中克隆获得2个γ-干扰素诱导的溶酶体巯基还原酶(GILT)基因AjGILT-1和AjGILT-2,并对其序列进行生物信息学分析,进而利用实时荧光定量PCR分别检测2个基因在不同组织器官中的表达水平,及其在脂多糖(LPS)、聚肌胞苷酸(PolyI:C)刺激和迟缓爱德华氏菌(Edwardsiella tarda)感染后的表达变化.结果显示:2个基因编码的蛋白序列中均含有GILT家族的特征序列CQHGX2ECX2NX4C、活性位点CXXC、2个糖基化位点和11个保守的半胱氨酸残基,基因组序列均含7个外显子和6个内含子.2个基因启动子中都存在多个转录因子结合位点,如γ-干扰素活化位点以及特化蛋白1结合位点; 不同的是AjGILT-1启动子区存在多个核因子-1、激活蛋白-1结合位点及干扰素刺激反应元件,而在AjGILT-2启动子区未发现.AjGILT-1在鳃和肠中表达量最高,而AjGILT-2在性腺、头肾和脾脏中表达量最高; 除肠道外,同一组织中AjGILT-2的表达量均高于AjGILT-1.LPS、PolyI:C刺激和E. tarda感染后,AjGILT-1与AjGILT-2的表达在鳃中仅有少数变化,而在头肾和中肾中则变化较明显.综上所述,AjGILT-1和AjGILT-2基因可能参与了日本鳗鲡受病毒和细菌感染后的免疫应答.
Abstract:
Two interferon-γ-inducible lysosomal thiol reductase(GILT)genes named AjGILT-1 and AjGILT-2 were cloned and identified from Anguilla japonica.The deduced AjGILT-1 and AjGILT-2 coding sequences contained the major characteristic features of the GILT protein family:the GILT signature sequence CQHGX2ECX2NX4C,the active site CXXC,two potential N-linked glycosylation sites and eleven conserved cysteines.The AjGILT-1 and AjGILT-2 contained seven exons separated by six introns.Moreover,AjGILT-1 and AjGILT-2 shared many transcription factor binding sites in the promoter region,such as interferon-γ activation site and serum response factor binding sites.The difference between them is that AjGILT-1 has nuclear factor-1,activator protein-1 binding sites and interferon stimulation reaction element,while AjGILT-2 does not.Quantitative real-time PCR analysis revealed that the expression level of AjGILT-1 was highest in gill and intestine,while the expression level of AjGILT-2 was highest in sex gonad,head kidney and spleen.The expression level of AjGILT-2 was higher than that of AjGILT-1 in most tissues,except for the intestine.After the stimulation with lipopolysaccharide(LPS),polyinosin-polycytidylic acid(PolyI:C)or infection with Edwardsiella tarda,there were only a few expression changes of AjGILT-1 and AjGILT-2 in the gills,while the expression changes in the head and middle kidneys were more obvious.In summary,AjGILT-1 and AjGILT-2 may be involved in immune responses to infection of viral and bacterial pathogens.

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备注/Memo

备注/Memo:
收稿日期:2018-03-14 录用日期:2018-04-16
基金项目:福建省自然科学基金(2016J06008和2018J01452)
*通信作者:wshuang@jmu.edu.cn
引文格式:陈姗,张芳芳,黄贝,等.日本鳗鲡γ-干扰素诱导的溶酶体巯基还原酶(GILT)基因的克隆鉴定与表达分析[J].厦门大学学报(自然科学版),2018,57(4):484-493.
Citation:CHEN S,ZHANG F F,HUANG B,et al.Cloning and expression analysis of interferon-γ-inducible lysosomal thiol reductase(GILT)gene in Anguilla japonica[J].J Xiamen Univ Nat Sci,2018,57(4):484-493.(in Chinese)
更新日期/Last Update: 1900-01-01