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[1]李小杭,郑年丰,韩守法*.一种用于细菌酸化成像的电势差响应探针[J].厦门大学学报(自然科学版),2018,57(04):455-462.[doi:10.6043/j.issn.0438-0479.201801020]
 LI Xiaohang,ZHENG Nianfeng,HAN Shoufa*.An Electropotential-responsive Probe for the Study of Bacterial Acidification-imaging[J].Journal of Xiamen University(Natural Science),2018,57(04):455-462.[doi:10.6043/j.issn.0438-0479.201801020]
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
57卷
期数:
2018年04期
页码:
455-462
栏目:
研究论文
出版日期:
2018-07-31

文章信息/Info

Title:
An Electropotential-responsive Probe for the Study of Bacterial Acidification-imaging
文章编号:
0438-0479(2018)04-0455-08
作者:
李小杭郑年丰韩守法*
厦门大学化学化工学院,福建 厦门 361005
Author(s):
LI XiaohangZHENG NianfengHAN Shoufa*
College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China
关键词:
细菌成像 酸化 pH探针 电势差响应
Keywords:
bacterial imaging acidification pH probe electric potential difference response
分类号:
O 657.3
DOI:
10.6043/j.issn.0438-0479.201801020
文献标志码:
A
摘要:
酸响应化学传感器已广泛用于哺乳动物细胞的成像,但目前缺乏用于研究酸性环境与细菌相互作用的细菌定位探针.为此,设计合成了一个电势差响应阳离子探针CM-RB,它由一个具有蓝色荧光的香豆素和一个带正电荷的酸敏感罗丹明结构单元构成.对CM-RB进行pH滴定、毒性测试及其在酸性环境下细菌内部的荧光响应研究.结果表明:CM-RB在细菌跨膜负电势的驱动下,聚集在细菌内部时产生蓝色荧光; 当细菌处于酸性条件下时,探针的酸敏感罗丹明结构单元产生红色荧光.CM-RB的活细菌靶向性及pH关联双色响应显示该探针具有用于细菌内部酸化过程分析的可行性.
Abstract:
Acidity-reporting chemosensors have been widely used in the imaging of mammalian cells,but there are currently lack of bacterial localization probes for studying the interaction between acidic environment and bacteria.In this study,an electropotential-responsive cationic probe CM-RB was designed,which was composed of a blue fluorescent coumarin unit and a positive acid sensitive rhodamine structure unit.The pH titration,toxicity test of CM-RB and the fluorescence response of bacteria in the acidic environment were studied.The results showed that CM-RB gathered inside of bacterial and generated blue fluorescence,when it was driven by the bacterial transmembrane negative potential; when bacteria were under acidic conditions,the acid sensitive rhodamine structure unit of probe produced red fluorescence.The living bacterial target and pH associated two-color response show that CM-RB has the feasibility of analyzing the acidification process of bacteria.

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备注/Memo

备注/Memo:
收稿日期:2018-01-22 录用日期:2018-03-22
基金项目:国家自然科学基金(21775130,21572189)
*通信作者:shoufa@xmu.edu.cn
引文格式:李小杭,郑年丰,韩守法.一种用于细菌酸化成像的电势差响应探针[J].厦门大学学报(自然科学版),2018,57(4):455-462.
Citation:LI X H,ZHENG N F,HAN S F.An electropotential-responsive probe for the study of bacterial acidification-imaging[J].J Xiamen Univ Nat Sci,2018,57(4):455-462.(in Chinese)
更新日期/Last Update: 1900-01-01