|本期目录/Table of Contents|

[1]熊丽霞,刘永亮,刘 洋,等.基于酶联免疫斑点技术的丙型肝炎病毒中和抗体检测平台的建立[J].厦门大学学报(自然科学版),2017,56(01):79-84.[doi:10.6043/j.issn.0438-0479.201602011]
 XIONG Lixia,LIU Yongliang,LIU Yang,et al.Establishment of Hepatitis C Virus Neutralizing Antibody Screening Platform Based on Enzyme-linked Immunospot Assay[J].Journal of Xiamen University(Natural Science),2017,56(01):79-84.[doi:10.6043/j.issn.0438-0479.201602011]
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基于酶联免疫斑点技术的丙型肝炎病毒中和抗体检测平台的建立(PDF)
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
56卷
期数:
2017年01期
页码:
79-84
栏目:
研究论文
出版日期:
2017-01-23

文章信息/Info

Title:
Establishment of Hepatitis C Virus Neutralizing Antibody Screening Platform Based on Enzyme-linked Immunospot Assay
文章编号:
0438-0479(2017)01-0079-06
作者:
熊丽霞12刘永亮13刘 洋13葛胜祥13*
1.厦门大学 分子疫苗学与分子诊断学国家重点实验室,国家传染病诊断试剂与疫苗工程技术研究中心,2.厦门大学生命科学学院,3.厦门大学公共卫生学院,福建 厦门 361102
Author(s):
XIONG Lixia12LIU Yongliang13LIU Yang13GE Shengxiang13*
1.State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics,National Institute of Diagnostics and Vaccine Development in Infectious Diseases,Xiamen University,2.School of Life Sciences,Xiamen University,3.School of Public Health,Xiamen Uni
关键词:
丙型肝炎病毒 酶联免疫斑点 中和抗体
Keywords:
hepatitis C virus(HCV) enzyme-linked immunospot assay(ELISPOT) neutralizing antibody
分类号:
Q 31
DOI:
10.6043/j.issn.0438-0479.201602011
文献标志码:
A
摘要:
为建立血清中丙型肝炎病毒(HCV)中和抗体检测及评价手段,运用HCV核心区特异性抗体17H11及链霉亲和素-生物素放大系统,建立了基于酶联免疫斑点(ELISPOT)技术的HCV中和抗体检测平台.结果显示,该平台计数点清晰,可实现阴阳性分开,并能有效地检测并评价HCV 抗体阳性病人血清的中和作用,表明该平台可实现HCV中和抗体的检测,将有利于HCV疫苗研制中的免疫效果评价.
Abstract:
To establish the detection and evaluation methods of hepatitis C virus(HCV)neutralizing antibody in serum,an enzyme-linked immunospot assay(ELISPOT)based platform was established for detection of HCV neutralizing antibody using HCV core specific antibody 17H11 and streptavidin-biotin amplification system.This platform shows clearly counted foci and differentiable signals between positive and negative samples,which can also effectively detect and evaluate the neutralization effect in patients with the positive HCV antibody reaction.This platform is proved as an efficient HCV neutralizing antibody detection method and will benefit the evaluation of immunity effect in HCV vaccine development.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-02-16 录用日期:2016-04-06
基金项目:国家重点基础研究发展计划(973计划)项目(2014CB560710)
*通信作者:sxge@xmu.edu.cn
更新日期/Last Update: 1900-01-01