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《厦门大学学报（自然科学版）》[ISSN:0438-0479/CN:35-1070/N]

卷:

56卷

期数:

2017年01期

页码:

3-12

栏目:

综 述

出版日期:

2017-01-23

文章信息/Info

Title:

The Reduced-representation Sequencing Technique and Its Applications in Marine Organisms

文章编号:

0438-0479(2017)01-0003-10

作者:

边 力; 王 军^*

厦门大学海洋与地球学院,福建 厦门 361102

Author(s):

BIAN Li; WANG Jun^*

College of Ocean & Earth Sciences,Xiamen University,Xiamen 361102,China

关键词:

简化基因组测序;  海洋生物;  基因分型;  限制性酶切位点相关DNA测序

Keywords:

reduced-representation sequencing;  marine organism;  genotyping;  restriction site-associated DNA sequencing(RAD-seq)

分类号:

Q 341

DOI:

10.6043/j.issn.0438-0479.201607015

文献标志码:

A

摘要:

传统开发遗传标记的方法通常会消耗大量的人力、物力和时间,伴随着高通量测序技术的飞速发展,一种高效的标记开发技术即简化基因组测序技术开始得到广泛应用.简化基因组测序技术可以在一次实验中获得成千上万的遗传标记,建库过程简易,成本较低.其通过实验手段降低基因组的复杂度,仅对部分基因组进行测序,随后在该部分获得的基因组开发分子标记.基于酶切的简化基因组测序技术可分为三大类:简化代表库测序、限制性酶切位点相关DNA测序和低覆盖度的分型测序.在海洋生物研究中,简化基因组测序技术已被广泛应用于群体遗传学、系统进化学、适应性进化、遗传图谱构建及数量性状位点定位等研究领域.

Abstract:

Traditionally,methods of molecular marker development were expensive and involved iterative,time-consuming PCR process.With the rapid development of next generation sequencing,a very efficient marker development technique,reduced-representation sequencing,has been widely utilized in various research areas.The library construction protocol is simple with low cost.More-over,the reduced-representation sequencing could identify thousands of markers in only one sequencing process.It reduces the complexity of the genome,sequences the reduced genome and searches markers on this partial genome.The reduced-representation sequencing can be grouped into three classes:reduced-representation sequencing,restriction site-associated DNA sequencing(RAD-seq),and low coverage genotyping.This technique has been widely applied in population genetics,phylogenetics,adaptive evolution,linkage map and quantitative trait locus(QTL)mapping studies in marine organisms.

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备注/Memo

备注/Memo:

收稿日期:2016-07-13 录用日期:2016-08-23
基金项目:国家自然科学基金(31372504; 41476118)
*通信作者:junw@xmu.edu.cn

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更新日期/Last Update: 1900-01-01