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[1]蒋庆茹,柯才焕,虞晋晋,等.杂色鲍肠道益生菌的分离和鉴定[J].厦门大学学报(自然科学版),2012,51(4):782.
 JIANG Qing ru,KE Cai huan,YU Jin jin,et al.Isolation and Characterisation of Putative Probiotics from Haliotis diversicolour[J].Journal of Xiamen University(Natural Science),2012,51(4):782.
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
51卷
期数:
2012年第4期
页码:
782
栏目:
研究论文
出版日期:
2012-07-15

文章信息/Info

Title:
Isolation and Characterisation of Putative Probiotics from Haliotis diversicolour
作者:
蒋庆茹柯才焕虞晋晋赵晶*
厦门大学海洋与地球学院,福建 厦门 361005
Author(s):
JIANG QingruKE CaihuanYU JinjinZHAO Jing*
College of Ocean and Earth Sciences,Xiamen University,Xiamen 361005,China
关键词:
杂色鲍益生菌消化酶抑菌安全性检测
Keywords:
Haliotis diversicolorprobioticsdigestive enzymes activitypathogen inhibitionsafety test
分类号:
Q 939.9
文献标志码:
-
摘要:
从杂色鲍(Haliotis diversicolor)肠道内,共分离出84株细菌,经16S rRNA 基因序列比对,共产生了35种类型(OTU),分属于17个属,利用纸片扩散法进行了细菌消化酶活性及抑菌活性检测.有75株菌在不同程度上表现出淀粉酶活性、蛋白酶活性、脂肪酶活性、琼胶酶活性、纤维素酶活性或褐藻酸钠酶活性;有24株菌不同程度地抑制2~8株致病菌的生长.结合实验结果,对9株潜在益生菌进行溶血实验检测及动物安全性实验.其中AP12和AP34不产生溶血素,并且在菌株浓度108 CFU/g时对鲍无明显的
Abstract:
The use of probiotics provides a potential alternative method in aquaculture.This study was designed to search for probiotics to improve growth rate and disease resistance of farmed abalone,Haliotis diversicolour.Potential probiotics were selected from the digestive tract of cultured abalones.The selection was based on five criteria: strain identification,digestive enzyme activity assay,pathogen inhibition potential,hemolytic activity assay and twomonth farm trials with putative probioticssupplemented diet.This study isolated 84 strains which were divided into thirtyfive operational taxonomic units (OTUs) and 17 genera,based on 16S rRNA gene sequencing.The results showed that 89% of screened bacteria (n=84) showed digestive enzyme activity.Only one isolate AP34 could use all the five substances (soluble starch,nonfat dry milk,glyceryltributyrate,CMcellulose,sodium alginate) provided as nutrition.24 out of 84 strains had antagonistic properties towards pathogens.Only one isolate AP12 could inhibit all the eight pathogens.Furthermore,both AP12 and AP34 showed no hemolytic activity,and the twomonth farm trials showed the two isolates were not harmful to cultured abalones.AP12 and AP34 have 99.87% and 98.63% 16S rRNA gene sequence similarities with Bacillus stratosphericus and Phaeobacter daeponensi,respectively.They have promising potential as probiotics for commercially produced H.diversicolour.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:20110912基金项目:福建省自然科学基金项目(2009J01191);福建省产业技术开发项目(闽发改投资(2010)983号)*通信作者:sunnyzhaoj@xmu.edu.cn
更新日期/Last Update: 2012-07-15