|本期目录/Table of Contents|

[1]熊裕焱,彭雅娟,卢瑞,等.地衣芽孢杆菌基因文库的构建及分析[J].厦门大学学报(自然科学版),2011,50(03):593.
 XIONG Yu yan,PENG Ya juan,LU Rui,et al.Construction and Analysis of Genomic Library of Bacillus licheniformis[J].Journal of Xiamen University(Natural Science),2011,50(03):593.
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地衣芽孢杆菌基因文库的构建及分析(PDF)
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
50卷
期数:
2011年03期
页码:
593
栏目:
研究论文
出版日期:
2011-05-20

文章信息/Info

Title:
Construction and Analysis of Genomic Library of Bacillus licheniformis
作者:
熊裕焱彭雅娟卢瑞于怡王娜李清彪何宁*
厦门大学化学化工学院,福建 厦门 361005
Author(s):
XIONG YuyanPENG YajuanLU RuiYU YiWANG Na LI QingbiaoHE Ning*
College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China
关键词:
地衣芽孢杆菌细菌絮凝基因基因文库
Keywords:
Bacillus licheniformisbacteriaflocculation genegenomic library
分类号:
Q 785
文献标志码:
-
摘要:
作为一种高效无毒、无二次污染以及能够生物降解的水处理剂,生物絮凝剂受到越来越多的关注.以一株具有强絮凝活性地衣芽孢杆菌(Bacillus licheniformis)为实验菌,通过构建基因组文库,尝试筛选絮凝基因阳性克隆子.结果表明,该菌株基因文库构建成功,共得到2.1×103克隆子.随机挑取17个阳性克隆子进行DNA测序分析,发现一些相对保守蛋白及2个不具有明确功能的基因.该结果为进一步研究地衣芽孢杆菌全基因组结构功能及细菌絮凝基因奠定了基础.
Abstract:
As an efficient,innoxious, and biodegradable wastewater treatment agent with no secondary pollution,bioflocculant is receiving more and more concerns nowadays.In this paper,Bacillus licheniformis with excellent flocculating activity was used to construct the genomic library and tried to screen out the positive clones with flocculation gene.The results showed that the genomic library was successfully constructed,2.1×103 clones were screened out. 17 clones were randomly selected for DNA sequencing and analysis.Some relative conserved proteins and 2 novel genes with unknown functions were found.The results lay a foundation for the further study of gene encoding bacteria flocculant and the whole genomic structure and function of Bacillus licheniformis.

参考文献/References:


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备注/Memo

备注/Memo:
收稿日期:20100720基金项目:国家自然科学基金项目(30700020);福建省发改委产业技术开发项目(闽发投资[2010]983号);厦门市科技计划项目(3502Z20093006)*通信作者:hening@xmu.edu.cn
更新日期/Last Update: 2011-05-20