|本期目录/Table of Contents|

[1]韦杨烨,徐岷涓,林文翰,等.Exfoliazone通过诱导细胞凋亡抑制HepG2细胞体外增殖[J].厦门大学学报(自然科学版),2010,49(03):423.
 WEI Yang ye,XU Min juan,LIN Wen han,et al.Exfoliazone Inhibits the Growth of HepG2Cells by Inducing Apoptosis[J].Journal of Xiamen University(Natural Science),2010,49(03):423.
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Exfoliazone通过诱导细胞凋亡抑制HepG2细胞体外增殖(PDF)
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《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
49卷
期数:
2010年03期
页码:
423
栏目:
研究论文
出版日期:
2010-05-20

文章信息/Info

Title:
Exfoliazone Inhibits the Growth of HepG2 Cells by Inducing Apoptosis
作者:
韦杨烨1徐岷涓2林文翰2邱彦3 张晓坤1曾锦章1*
1.厦门大学生物医学研究院,福建 厦门 361005;2.北京大学药学院天然药物与仿生药物国家重点实验室,北京100083; 3.厦门大学医学院,福建 厦门 361005
Author(s):
WEI Yangye1XU Minjuan2LIN Wenhan2QIU Yan3 ZHANG Xiaokun1ZENG Jinzhang1*
1.Institute for Biomedical Research,Xiamen University,Xiamen 361005,China;2.State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Science,Peking University,Beijing 100083,China; 3.Medical College,Xiamen University,Xiamen 361005,C
关键词:
海洋药物Exfoliazone肝癌凋亡
Keywords:
marine drugExfoliazoneliver cancerapoptosis
分类号:
R 963
文献标志码:
-
摘要:
探讨Exfoliazone对肝癌细胞HepG2增殖及凋亡的影响.采用细胞培养技术,用不同浓度的药物在一定的时间内处理细胞株,应用MTT比色法检测Exfoliazone对HepG2细胞体外生长的抑制作用;4′,6二脒基2苯基吲哚(DAPI)荧光染料染色,显示凋亡细胞形态;用WesternBlotting检测聚腺苷二磷酸核糖聚合酶(PARP)蛋白的表达;Annexin V/PI双染检测Exfoliazone对HepG2细胞凋亡的影响.Exfoliazone可抑制HepG2细胞的生长,并呈现剂量和时间的抑制,在药物处理24、48和72 h后,半数细胞抑制浓度(IC50)分别为35、16 和8 μmol/L.进一步研究表明,Exfoliazone可诱导PARP切割,细胞出现核质浓集和凋亡小体等典型的凋亡形态学特征,Annexin V/PI染色证实Exfoliazone能诱导HepG2细胞凋亡,并且以早期凋亡为主.Exfoliazone可能通过诱导HepG2细胞凋亡抑制癌细胞的生长.
Abstract:
This study is to investigate the antiproliferation effect of Exfoliazone on liver cancer cell line HepG2 by inducing apoptosis.The test of antiproliferation effect was performed by MTT assay,apoptotic cells were identified by DAPI staining and AnnexinV/PI staining.The expression of the poly ADPribose polymerase protein and its activated fragment were detected by WesternBlotting assays.Exfoliazone treatment significantly inhibited the growth of HepG2 cells in a time and dosedependent manner.The IC50 for Exfoliazone was about 35,16 and 8 μmol/L after treatment for 24,48 and 72 h,respectively.Exfoliazone treatment could greatly induce apoptosis of HepG2 as indicated by DAPI staining,PARP cleavage and AnnexinV/PI staining.Exfoliazoneinhibits the growth of HepG2 cells by inducing apoptosis.

参考文献/References:


[1]谢渭芬,蔡洪培.原发性肝癌的治疗进展[J].中国实用内科杂志:临床前沿版,2006(12):19311933.
[2]Zhang J Y.Apoptosisbased anticancer drugs[J].Nat Rev Drug Discov,2002,1:101102.
[3]Reed J C.Apoptosisbased therapies[J].Nat Rev Drug Discov,2002,1:111121.
[4]Schwartsmann G,Ratain M J,Cragg G M,et al.Anticancer drug discovery and development throughout the world[J].J Clin Oncol,2002,20:4759.
[5]黄丽波,徐石海,岑颖洲.海绵中抗肿瘤抗病毒生物活性的生物碱研究进展[J].广州医药,2001,32(1):325.
[6]刘家峰,郭松坡,姜标.海洋溴吡咯生物碱的研究进展[J].有机化学,2005,25(7):788799.
[7]Shinsuke I,Tadashi N,Haruo S,et al.Exfoliazone and lavanducyanin,potent growth promoting substances of rat liver cell line,RLN8,produced by streptomyces exfoliatus and streptomyces aeriouvifer[J].J Antibiot,1993,46(8):12321238.
[8]Yuan J H,Zhang R P,Zhang R G,et al.Growthinhibiting effects of taxol on human liver cancer in vitro and in nude mice[J].World J Gastroenterol,2000,6(2):210215.
[9]Ashkenazi A,Dixit V M.Death receptors:signaling and modulation[J].Science,1998,281(5381):13051308.
[10]Reed J C.Bcl2 family proteins[J].Oncogene,2002,17:32253236.
[11]Liu J,Zhou W,Li S S,et al.Modulation of orphan nuclear receptor Nur77mediated apoptotic pathway by acetylshikonin and analogues[J].Cancer Res,2008,68(21):88718880.

备注/Memo

备注/Memo:
收稿日期:20091028基金项目:国家863项目(2007AA09Z404);国家自然科学基金项目(30971445,30931100431)*通讯作者:jzzeng@xmu.edu.cn
更新日期/Last Update: 2010-05-20