|本期目录/Table of Contents|

[1]苏仲毅,陈猛*,袁东星,等.固相萃取超高压液相色谱串联质谱法分析海水中9种磺胺类抗生素[J].厦门大学学报(自然科学版),2007,46(S1):72.
 SU Zhong yi,CHEN Meng*,YUAN Dong xing,et al.Determination of Nine Sulfonamide Antibiotics in Seawater Using Solid Phase Extraction and Ultra Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry[J].Journal of Xiamen University(Natural Science),2007,46(S1):72.
点击复制

固相萃取超高压液相色谱串联质谱法分析海水中9种磺胺类抗生素(PDF)
分享到:

《厦门大学学报(自然科学版)》[ISSN:0438-0479/CN:35-1070/N]

卷:
46卷
期数:
2007年S1期
页码:
72
栏目:
环境化学
出版日期:
2007-12-15

文章信息/Info

Title:
Determination of Nine Sulfonamide Antibiotics in Seawater Using Solid Phase Extraction and Ultra Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry
作者:
苏仲毅陈猛*袁东星游明华
厦门大学环境科学研究中心,福建 厦门 361005
Author(s):
SU ZhongyiCHEN Meng*YUAN DongxingYOU Minghua
Environmental Science Research Center,Xiamen University,Xiamen 361005,China
关键词:
磺胺海水固相萃取液相色谱质谱
Keywords:
sulfonamideseawatersolid phase extractionliquid chromatographymass spectrometryX.
分类号:
O 657.63
文献标志码:
-
摘要:
应用固相萃取及液相色谱质谱联用技术,建立了海水中9种磺胺类抗生素的分析方法.海水样品经HLB固相萃取柱富集、净化后用甲醇洗脱浓缩定容,利用超高压液相色谱串联质谱仪多反应监测(MRM)离子模式对目标物进行定性、定量分析.9种磺胺测定的线性范围为0.2~1 000 μg/L,方法检测限在0.2~0.4 ng/L之间(S/N=10,1 000倍浓缩).以海水为基底,磺胺甲基嘧啶为替代物,加标浓度分别为5.0 ng/L和20.0 ng/L时,9种磺胺的回收率在86.4%~150.4%之间,相对标准偏差0.5%~8.4%(n=4),方法成功应用于厦门近岸海域海水样品中抗生素残留的分析.
Abstract:
A method for simultaneous determination of 9 sulfonamides (sulfadiazine,sulfathiazole,sulfapyridine,sulfamethazine,sulfameter,sulfamethoxazole,sulfamonomethoxine,sulfadimethoxine and sulfachinoxaline) in seawater was developed.The target analytes were extracted on a porous polymer Oasis HLB column and subsequently eluted with methanol.Qualitative and quantitative analysis was carried out on a Waters ultra performance liquid chromatography (UPLC) with electrospray tandem mass spectrometry(ESIMSMS) using multireactive monitoring mode.Good linearity was obtained in the range of 0.2~1 000 μg/L.The method detection limits varied between 0.2 ng/L and 0.4 ng/L(S/N=10,concentration factor=1 000).Recoveries were between 86.4%~150.4% in seawater matrix at both 5.0 ng/L and 20.0 ng/L spiking levels using sulfamerazine as a surrogate,the relative standard deviations (RSD,n=4) were in the range of 0.5%~8.4%.The method has been successfully applied in the survey of sulfonamide residue in the seawater samples collected from Xiamen area.

参考文献/References:


[1]Alistairb A B,Danaw K,Bent H,et al.Are veterinary medicines causing environmental risks[J].Environmental Science and Technology,2003,August 1:287293.
[2]胡莹莹,王菊英,冯德毅.近岸养殖区抗生素的海洋环境效应研究进展[J].海洋环境科学,2004,23(4):77-79.
[3]刘玉春,徐维海,余莉莉,等.固相萃取液相色谱质谱/质谱联用测定河水中大环内酯类抗生素[J].分析测试学报,2006,25(2):1-5.
[4]Zhu J,Snow D D,Cassada D A,et al.Analysis of oxytetracycline,tetracycline,and chlortetracycline in water using solidphase extraction and liquid chromatographytandem mass spectrometry[J].J Chromatography A,2001,928:177-186.
[5]Angela L B,Ian B B,Diana S A.Evaluating the vulnerability of surface waters to antibiotic contamination from varying wastewater treatment plant discharges[J].Environmental Pollution,2006,142:295-302.
[6]Shinwoo Y,Kenneth C.Evolution of antibiotic occurrence in a river through pristine,urban and agricultural landscapes[J].Water Research,2003,37:4645-4656.
[7]Xu W H, Zhang G, Zou S,et al.Determination of selected antibiotics in the Victoria Harbour and the Pearl River,South China using highperformance liquid chromatographyelectrospray ionization tandem mass spectrometry[J].Environmental Pollution,2007,145:672-679.
[8]Sandra R,Francesc B,Eva P,et al.Determination of antibiotic compounds in water by solidphase extractionhigh performance liquid chromatography(electrospray) mass spectrometry[J].J Chromatography A,2003,1010:225-232.
[9]Xiu S,Farida B,Chen M,et al.Occurrence of antimicrobials in the final effluents of wastewater treatment plants in Canada[J].Environmental Science and Technology,2004,38:3533-3541

备注/Memo

备注/Memo:
收稿日期:20070505基金项目:福建省自然科学基金(2006J0140)资助*通讯作者:mengchen@xmu.edu.cn
更新日期/Last Update: 2007-06-20