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Therapeutic Effect of Bone Marrow Mesenchymal Stem Cells on Experimental Allergic Encephalomyelitis in Mice(PDF)

Journal of Xiamen University(Natural Science)[ISSN:0438-0479/CN:35-1070/N]

Issue:
2017 04
Page:
606-610
Research Field:
Research Notes
Publishing date:
2017-07-26

Info

Title:
Therapeutic Effect of Bone Marrow Mesenchymal Stem Cells on Experimental Allergic Encephalomyelitis in Mice
Article ID:
0438-0479(2017)04-0606-05
Author(s):
JIA Chunfeng1*SHAO Lin2DAI Qingchen1LE Lisheng2
1.Department of Orthopedics,Fuzhou Second Hospital,Fuzhou 350007,China; 2.Fuzhou Economic and Technological Development Zone Hospital,Fuzhou 350007,China
Keywords:
allergic encephalomyelitis bone mesenchymal stem cells(BMMSCs) inflammatory cytokines
CLC number:
R-33
DOI:
10.6043/j.issn.0438-0479.201604056
Document code:

A
Abstract:
To study the treatment effects of bone mesenchymal stem cells(BMMSCs)on the mice allergic encephalomyelitis,36 mice were randomly divided into three groups(control group,model group and BMMSCs group),12 for each group,and treated with different methods.After treatment for 8 weeks,expressions of IL-1,IL-2,IL-4,IL-6,IL-10,IL-12,TNF-α and IFN-γ of spinal cord tissues in mice were analyzed by RT-PCR and Western blot,and HE staining were performed to observe morphological changes of spinal cord tissues in mice.The RT-PCR and Western blot results showed as follows:compared to the control group,mRNA and protein levels of IL-1,IL-2,IL-12,TNF-α and IFN-γ significantly increased(p<0.05),while those of IL-4 and IL-10 significantly decreased(p<0.05)in the model group.In the BMMSCs group,the mRNA and protein levels of IL-1,IL-2,IL-12,TNF-α,IFN-γ were significantly downregulated(p<0.05),while those of IL-4 and IL-10 were significantly upregulated(p<0.05), compared with the model group.The HE staining results showed as follows:the structure of the spinal cord tissues and morphology of nerve cells were intact in the control group; in the model group,abundant inflammatory cells appeared in the spinal cord tissues; in contrast,no obvious inflammatory cells were observed in the BMMSCs group.Taking together,it is suggested that BMMSCs have therapeutic effect on experimental allergic inflammatory response in mice,and the mechanism involves inhibiting the expressions of inflammatory cytokines and reduce the damage to the spinal cord.

References:

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Memo

Memo:
收稿日期:2016-04-28 录用日期:2016-08-15
基金项目:福州市科技计划项目(2015-S-141-18)
*通信作者:jiachunfeng_fj@126.com
Last Update: 1900-01-01