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Biodiversity Analysis and Bioactivity Detection of Actinomycetes from Rhizosphere Soil in Xiamen and Nanjing Area,China(PDF)

Journal of Xiamen University(Natural Science)[ISSN:0438-0479/CN:35-1070/N]

2017 04
Research Field:
Research Articles
Publishing date:


Biodiversity Analysis and Bioactivity Detection of Actinomycetes from Rhizosphere Soil in Xiamen and Nanjing Area,China
Article ID:
WANG Fei1LI Dan1HUANG Yaojian1DENG Xianming1WU Yingying2*
1.State-Province Joint Engineering Laboratory of Targeted Drugs from Natural Products,School of Life Sciences,Xiamen University,Xiamen 361102,China; 2.National Engineering Research Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shan
actinomycetes selective isolation biodiversity bioactivity
CLC number:
Q 939
Document code:

To investigate the diversity of actinomycetes from rhizosphere soil in different regions and to screen the strains with antibacterial and antitumor activities,the 16S rRNA gene sequencing and phylogenetic analysis were used to explore the diversity of actinomycetes,which were isolated from 17 rhizosphere soil samples with two selective media.And then 6 bacteria,2 fungi and 4 tumor cells were used as indicators to determine the biological activities of actinomycetes.In this study,a total of 178 actinomycetes were isolated from the rhizosphere soil,including 27 strains as rare actinomycetes,which accounted for a total of 15.2%.The rare actinomycetes can be assigned to 9 genera,which are Microbacterium,Amycolatopsis,Kribbella,Nonomuraea,Micromonospora,Streptosporangium,Kibdelosporangium,Cellulosimicrobium,Isoptericola,and 2 strains of them were novel species.Among the isolated actinomycetes,82 strains(46.1%)had antibacterial activities against one or more indicators,60 strains(33.7%)had inhibitory activities against one or more tumor cells.In conclusion,the results show that the rhizosphere soil is a highly diverse actinobacterial community,in which most of them appear to be novel candidate species.Therefore,the actinomycetes with biological activities could be a promising source for secondary metabolites separation and microbial pharmaceutical developments.


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收稿日期:2016-06-07 录用日期:2016-09-02
基金项目:国家自然科学基金(U1405223); 福建省教育厅科技项目(JA15020)
Last Update: 1900-01-01